This chapter described the advancements in development of nanostructured supported material and enzyme immobilization techniques. The functionalized nanomaterials extremely affect inherent mechanical properties provide highest biocompatibility specific nano-environment surrounding enzymes for improving stability, catalytic performance, reaction’s activities. on considerably enhances robustness ...

BACKGROUND β-Galactosidase is a vital enzyme with diverse application in molecular biology and industries. It was covalently attached onto functionalized graphene nano-sheets for various analytical applications based on lactose reduction. METHODOLOGY/PRINCIPAL FINDINGS Response surface methodology based on Box-Behnken design of experiment was used for determination of optimal immobilization c...

The entrapment of enzymes in organic, inorganic or biomaterials can yield active immobilized biocatalysts for chemical transformations without the need to form covalent bonds with protein.

Background; Enzymes are well known as sensitive catalysts in the laboratory and industrial scale. To improve their properties and for using their significant potential in various reactions as a useful catalyst the stability of enzymes can often require improvement. Enzymes Immobilization on solid supports such as epoxy- functionalized ferric silica nanocomposite can be effective way to improve ...

The glucose oxidase (GOx) enzyme was immobilized on chitosan-based porous composite membranes using a covalent bond between GOx and the chitosan membrane. The chitosan-based porous membranes were prepared by the combination of the evaporation- and non-solvent-induced phase separation methods. To increase the membrane conductivity, carbon nanotubes (CNTs) were added to the chitosan solution. The...

In this paper, the stabilization of a lipase from Bacillus thermocatenulatus (BTL2) by a new strategy is described. First, the lipase is selectively adsorbed on hydrophobic supports. Second, the carboxylic residues of the enzyme are modified with ethylenediamine, generating a new enzyme having 4-fold more amino groups than the native enzyme. The chemical amination did not present a significant ...

In this work, a simple method for alcohol synthesis with high enantiomeric purity was proposed. For this, colloidal gold and silver surface modifications with 3-mercaptopropanoic acid and cysteamine were used to generate carboxyl and amine functionalized gold and silver nanoparticles of 15 and 45 nm, respectively. Alcohol dehydrogenase from Thermoanaerobium brockii (TbADH) and its cofactor (NAD...

Urease (urea amidohydrolase, EC 3.5.1.5), widely distributed in nature, catalyzes the hydrolysis of urea to ammonia and carbon dioxide at a rate 1014 times faster than the uncatalyzed reaction.1–3 Among enzymes, urease is most extensively studied for immobilization due to the possible exploitation in practical applications, such as quantitative determination of blood urea in clinical examinatio...