A new affinity method for quantification of glycated albumin by an enzyme-linked boronate-immunoassay (ELBIA) has been established, based on the interaction between boronic acids and the cis-diols of glycated human serum albumin (HSA) trapped by anti-HSA antibody. To evaluate the ELBIA, we first examined the accuracy of the conventional boronate affinity chromatographic (BAC) method. In the BAC...

Bredlg and von Bemeck,* in their work on inorganic ferments, determined the catalytic activity of platinum and manganese salts on hydrogen peroxide by means of titration with potassium permanganate. In many of their experiments they found, however, that the reaction velocity increased during the reaction. Bredig and Marck 2 attempt to explain this fact by a gradual activation of the catalyzer b...