Biochemical and molecular characterization of the biotin biosynthetic pathway in plants has dealt primarily with biotin synthase. This enzyme catalyzing the last step of the pathway is localized in mitochondria. Other enzymes of the pathway are however largely unknown. In this study, a genomic-based approach allowed us to clone an Arabidopsis (Arabidopsis thaliana) cDNA coding 7-keto-8-aminopel...

It is well-known that enzymes is very important as reaction factor in life systems activity. But the properties based on information theory are not yet enough in biological studies. Then, we examined correlation the complexity at amino acid sequences with its function of Enzymes by informational measure, in order to elucidate the informational properties of sequence structure. Also, power spect...

Förster resonance energy transfer (FRET) has been used to show that the average distance between proteins decreases when the protein is immobilized within polymer scaffolds, indicating that the immobilization process is inducing aggregation.

Photoaging causes the dermis and collagen fibers to decrease which results in skin aging. Genus Bacillus has SOD activity as an anti-photoaging agent that can neutralize ROS (Reactive Oxygen Species) due exposure UV-A rays. This study aimed examine of crude extract encapsulation characteristics liposomes from B. altitudinis. The began with isolation enzyme, determination total protein content e...

The importance of recycling and utilization of recycled fiber has recently been recognized because of concerns about environmental issues and economic aspects. In spite of several advantages offered by paper recycling such as wood savings through substitution of virgin pulp and reduction in chemical usage, recycled pulp also contains plenty of fines having high relative surface area and hig...

Background: Benzaldehyde dehydrogenase (BZDH) is encoded by the xylC that catalyzes the conversion of benzaldehyde into benzoate in many pathways such as toluene degradation. Objectives: In this study, the xylC gene from Rhodococcus ruber UKMP-5M was expressed in Escherichia coli, purified, and characterized.Materials and Methods: The xylC was amplified and cloned in E. coli. The re...

Background: Differentiation, migratory properties and availability of Mesenchymal Stromal Cells (MSC) have become an important part of biomedical research. However, the functional heterogeneity of cells derived from different tissues has hampered providing definitive phenotypic markers for these cells. Objective: To characterize and compare the phenotype and cytokines of adipose derived MSCs (...

(Cell Reports 26, 3741–3751.e1–e5; March 2019) In our paper, we reported the structure and biochemical characterization of RNA-guided RNase Cas13b from Prevotella buccae (PbuCas13b). This work included an assessment turnover rate this enzyme. Recently, it was brought to attention by Juan Santiago Ashwin Ramachandran that inconsistent with data (Figure 5D). Upon re-evaluation raw data, found err...