Abstract: Aim: To investigate chemo-preventive effect of methanolic fruit extract Cucumis melo var. agrestis on DEN (diethyl-nitrosamine) induced hepatocellular carcinoma in Sprague Dawley rats. Materials and Methods: Five groups rats randomly selected comprising 6 animals each group, all groups, except Group-1 were administered single dose (200 mg/kg, i.p. dissolved PBS) for the induction HCC ...

to determine the extent and the size of the environment pollution with heavy metals, biologicalmethods, investigating the enzymatic activity of soil can be applied. most frequently, the enzymes investigated in the soils threatened with anthropogenic contamination are phosphatases, dehydrogenases as reacting fastest to the increase in the content of heavy metals in the environment. the aim of th...

background: in patients with non-obstructive azoospermia (noa), vital spermatozoa from the tissue is obtained from testes by enzymatic treatment besides the mechanical treatment. objective: to increase the sperm recovery success of testicular sperm extraction (tese), with enzymatic digestion if no sperm is obtained from testis tissue by mechanical method. materials and methods: tissue samples w...

A relationship between the preexponent of the rate constant and the distribution over activation barrier energies for enzymatic/protein reactions is revealed. We consider an enzyme solution is an ensemble of individual molecules with different values of the activation barrier energy described by the distribution. From solvent viscosity effect on the preexponent we derive the integral equation f...

The role of seemingly non-enzymatic proteins in complexes interconverting UDP-arabinopyranose and UDP-arabinofuranose (UDP-arabinosemutases; UAMs) in the plant cytosol remains unknown. To shed light on their function, crystallographic and functional studies of the seemingly non-enzymatic UAM2 protein from Oryza sativa (OsUAM2) were undertaken. Here, X-ray diffraction data are reported, as well ...

This study was conducted to determine whether the application of high hydrostatic pressure could modify the enzymatic activity and membrane integrity of lysosomes in muscle. Several combinations of pressure (0-600 MPa) and time (0-300 s) were applied to two types of samples: purified enzymes (cathepsin D and acid phosphatase) in buffer solution and intact muscle (biceps femoris). The enzymes st...

This work shows the effect of 2 and 5 wt% TiO2 nanoparticles (modified by fluorocarbons and not) and of 1, 3 and 5 wt% ZnO particles on PLA degradation evaluated under UV irradiation, hydrolytical 1N NaOH solution, enzymatical proteinase K solution, and isothermal condition. The presence of TiO2 nanoparticles and ZnO particles decreases the degradation processes of PLA under UV light exposure a...

The preparation and characterization of UV-cured polyurethane-based materials for the mild inclusion immobilization of enzymes was investigated. Full curing of the polymer precursor/enzyme solution mixture was realized by a short irradiation with UV-light at ambient temperatures. The included aqueous enzyme solution remains highly dispersed in the polymer material with an even size distribution...

In this study, we demonstrated that microwave-assisted enzymatic digestion could be greatly accelerated by multifunctional magnetite beads. The acceleration of microwave-assisted enzymatic digestion by the presence of the magnetite beads was attributable to several features of the beads. Their capacity to absorb microwave radiation leads to rapid heating of the beads. Furthermore, their negativ...

Enzymatic fuel cells produce electrical power by oxidation of renewable energy sources. An enzymatic glucose biofuel cell uses glucose as fuel and enzymes as biocatalyst, to convert biochemical energy into electrical energy. The applications which need low electrical voltages and low currents have much of the interest in developing enzymatic fuel cells. An analytical modelling of an enzymatic f...