Forty-nine typical and atypical enteropathogenic Escherichia coli (EPEC) strains belonging to different serotypes and isolated from humans, pets (cats and dogs), farm animals (bovines, sheep, and rabbits), and wild animals (monkeys) were investigated for virulence markers and clonal similarity by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). The virulence marker...

aim : the purpose of this study was to investigate the prevalence of enteropathogenic escherichia coli (epec) and shiga toxin producing e. coli (stec) strains in healthy broilers in iran.   background : stec and epec strains as diarrheagenic e. coli are among the most prevalent causative agents in acute diarrhea. domestic animals, mainly cattle and sheep, have been implicated as the principal r...

background and objectives: enteropathogenic escherichia coli (epec) strains can be detected by serogrouping and the presence of enterocyte attaching- effacing (eae) gene. most epec strains belong to a certain o antigenic group. locus of enterocyte effacement (lee) pathogenicity island contains the eae gene and secretory proteins (esps) that introduce the attaching-effacing lesion. lee inserted ...

Enteropathogenic Escherichia coli (EPEC) and Shiga toxin-producing E. coli (STEC) are pathovars of E. coli that impact human health by causing childhood diseases. In this work, 118 synanthropic rodents of the genus Rattus from Buenos Aires, Argentina were evaluated as EPEC and STEC carriers. Rectal swab samples from captured animals were evaluated by conventional PCR to detect the presence of t...

The attaching effacing (AE) adherence property is now recognized as an important virulence characteristic of enteropathogenic Escherichia coli (EPEC). The fluorescent-actin staining (FAS) test (S. Knutton, T. Baldwin, P. H. Williams, and A. S. McNeish, Infect. Immun. 57:1290-1298, 1989), which is diagnostic for the AE lesions produced by EPEC (and Vero cytotoxin-producing E. coli), has provided...

Emerging Infectious Diseases • www.cdc.gov/eid • Vol. 12, No. 8, August 2006 1299 9. Adu-Bobie J, Frankel G, Bain C, Gonçalves AG, Trabulsi LR, Douce G, et al. Detection of intimin alpha, beta, gamma, and delta, four intimin derivatives expressed by attaching and effacing microbial pathogens. J Clin Microbiol. 1998;36:662–8. 10. Carvalho VM, Gyles CL, Ziebell K, Ribeiro MA, Catão-Dias JL, Sinho...

Abstract Background The diagnostics of travellers’ diarrhoea (TD) has been revolutionised by multiplex qPCR assays. While mostly bacterial aetiology, viruses and parasites account for the disease among 10–20% travellers. Despite this, prospective studies applying assays remain scarce that cover not only bacteria, such as various diarrhoeagenic Escherichia coli (DEC), but also viral parasitic pa...

BACKGROUND Integrons are the major reasons of multidrug resistance (MDR) among enteropathogenic bacteria. Occurrence of horizontal gene transfer between integron-carrying microorganisms and other enteric bacteria may increase the rate of emergence of integron-associated antibiotic resistance. OBJECTIVES The objective of this study was to investigate class 1 integrons among members of enteropa...

Both enteropathogenic Escherichia coli (EPEC) and an obligate intracellular bacterium, previously referred to as an intracellular Campylobacter-like organism and now designated Lawsonia intracellularis, have been reported as causes of enterocolitis in rabbits. An outbreak of enterocolitis in a group of rabbits, characterized by an unusually high rate of mortality, was found to be associated wit...

background and objectives: the therapeutic options for diseases caused by escherichia coli are limited. in this study we investigated the presence of virulence factors among enteropathogenic escherichia coli (epec) strains and their antibiotic resistance patterns. the isolates were also checked for the presence of class1 integrons and gene cassettes. materials and methods: this study included 7...