Chaetomium erraticum was capable of producing all the three components of a cellulase enzyme system including exoglucanase, endoglucanases, and β-glucosidase extracellularly. However, the cultivation conditions and the medium composition markedly affected the ability of microorganism to synthesize various enzymes. Exoglucanase was highest under static conditions, while endoglucanase and β-gluco...

was purified from extracts of plasmid-bearing E. coli cells by heat treatment and chromatography on DEAE-Trisacryl. It was characterized as a thermophilic endo-4-1,4-glucanase, the properties of which closely resemble those of endoglucanase A previously isolated from C. thermocellum supernatants. On sodium dodecyl sulfate-polyacrylamide gel electrophoresis the enzyme purified from E. coli exhib...

Endoglucanase is an important enzyme participating in the hydrolysis of cellulose into oligosaccharides or glucoses can be used a variety industrial fields. A gene (code GL0183420 designated as eg9) 1398 bp encoding for endoglucanase family GH8 was identified from metagenomic DNA data bacteria humus surrounding wood hydrolyzed by white rot fungi. The 126 terminal nucleotides at 5' encode signal...

Aspergillus nidulans is poorly exploited as a source of enzymes for lignocellulosic residues degradation for biotechnological purposes. This work describes the A. nidulans Endoglucanase A heterologous expression in Pichia pastoris, the purification and biochemical characterization of the recombinant enzyme. Active recombinant endoglucanase A (rEG A) was efficiently secreted as a 35 kDa protein ...

Degradative enzymes have been used to obtain defined fragments of the isolated cell walls of suspension-cultured sycamore cells. These fragments have been purified and structurally characterized. Fragments released from endopolygalacturonase-pretreated cell walls by a purified endoglucanase and the fragments extracted from these walls by urea and alkali provide evidence for a covalent connectio...

Reaction conditions for the reducing-end-specific derivatization of cellulose substrates with the fluorogenic compound, anthranilic acid, have been established. Hydrolysis of fluorescence-labelled celluloses by cellobiohydrolase Cel7A from Trichoderma reesei was consistent with the active-site titration kinetics (burst kinetics), which allowed the quantification of the processivity of the enzym...

Degradation of cotton cellulose by Trichoderma reesei endoglucanase I (EGI) and cellobiohydrolase II (CBHII) was investigated by analyzing the insoluble cellulose fragments remaining after enzymatic hydrolysis. Changes in the molecular-size distribution of cellulose after attack by EGI, alone and in combination with CBHII, were determined by size exclusion chromatography of the tricarbanilate d...

BACKGROUND Many agricultural and industrial food by-products are rich in cellulose and xylan. Their enzymatic degradation into monosaccharides is seen as a basis for the production of biofuels and bio-based chemicals. Lytic polysaccharide monooxygenases (LPMOs) constitute a group of recently discovered enzymes, classified as the auxiliary activity subgroups AA9, AA10, AA11 and AA13 in the CAZy ...

in this paper detection of endoglucanase gene and protein profile belonging to two fungal species, penicillium oxalicum 1sms and trichodermaviride156ms with high cellulotic activity was investigated. fungi isolates were cultured on inducer cmc medium and then released sugar and protein were assayed every three days for a month using arsenate molybdate reagent and bradford method, respectively. ...