The mediators in activating neural stem cells during the regenerative process of neurogenesis following stroke have not been fully identified. Milk fat globule-EGF Factor VIII (MFG-E8), a secreted glycoprotein serves several cellular functions by binding to its receptor, αv β3-integrin. However, its role in regulating neural stem cells after stroke has not been determined yet. We therefore, aim...

the cellular therapy and nerve tissue engineering will probably become a major therapeutic strategy for promoting axonal growth through injured area in central nervous system and peripheral nervous system in the coming years. the stem cell carrier scaffolds in nerve tissue engineering resulted in strong survival of cells and suitable differentiation into neural cells, so this pathway should be ...

Traumatic brain injury (TBI) leading to 5 million deaths annually is 1 of the 5 major causes of morbidity and mortality worldwide. In Iran, accidents are the main cause of death in youth as well as a dominant factor in reducing quality of life. In developing countries TBI incidence as one of the worst consequences of these accidents is growing due to wide use of motor-vehicles. Therapeutic stra...

multipotent skin-derived stem cell, and a purified neural stem cell from the brain have come on the scene at a delicate time in the debate over embryonic stem cell research. Each success in isolating adult stem cells, such as these cells, gives ammunition to opponents of embryonic stem cell research, who point to adult-derived stem cells as an alternative source of transplant-ready material. Th...

1. The embryonic neural stem cell (NSC) microenvironment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 1.1. The VZ microenvironment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 1.2. Signalling in the embryonic NSC microenvironment . . . . . . . . . . . . . . . . . . . . . . . . . . . ....

the aim of this study was to isolate mouse embryonic stem cells from late blastocyst stage embryos and to use them as a model system for the study of hematopoietic induction outside the embryo by coculturing of embryonic stem cells with bone marrow stromal cells. blastocyst stage embryos from pregnant nmri mice were obtained and cultured for 1-2 days in dmem medium. the inner cell masses formed...

Adult neural stem cells are generated at embryonic stages by entering a quiescent state that allows their retention into adulthood and thereby maintenance of life-long brain homeostasis. Thus, a tight balance between the quiescence and activation state is instrumental to meet the brain demands for a specific cell type at the correct numbers, at a given time and position. Protein synthesis is th...

Neural stem cells are reported to lie in a vascular niche, but there is no direct evidence for a functional relationship between the stem cells and blood vessel component cells. We show that endothelial cells but not vascular smooth muscle cells release soluble factors that stimulate the self-renewal of neural stem cells, inhibit their differentiation, and enhance their neuron production. Both ...