t d t d b SUMMARY 1. Physicians should inform cancer patients about options for fertility preservation and future reproduction prior to treatment. 2. The only established methods of fertility preservation are sperm cryopreservation in men and embryo cryopreservation in women. 3. Experimental procedures such as oocyte or ovarian tissue cryopreservation should be offered only in a research settin...

This study evaluates a new synthetic substitute (CRYO3, Ref. 5617, Stem Alpha, France) for animal-based products in rabbit embryo cryopreservation solutions. This evaluation was performed using two approaches: a thermodynamic approach using differential scanning calorimetry and a biological approach using rabbit embryo slow-freezing. During the experiment, foetal calf serum (FCS) was used as a ...

t d t d b SUMMARY 1. Physicians should inform cancer patients about options for fertility preservation and future reproduction prior to treatment. 2. The only established methods of fertility preservation are sperm cryopreservation in men and embryo cryopreservation in women. 3. Experimental procedures such as oocyte or ovarian tissue cryopreservation should be offered only in a research settin...

Between May 1996 and May 1998, 64 azoospermic patients underwent an investigative testicular biopsy combined with the cryopreservation of spermatozoa which were retrieved from a simultaneously examined fresh sample. Testicular tissue cryopreservation was carried out in 43 cases (67%) for late intracytoplasmic sperm injection (ICSI) attempts. In all, 23 couples underwent 26 assisted conception c...

OBJECTIVE To evaluate if in vitro fertilization (IVF) with embryo cryopreservation can be proposed to patients immediately after one or two regimens of chemotherapy. DESIGN Retrospective study. SETTING Academic research center and IVF unit. PATIENT(S) Eleven young patients diagnosed with cancer between September 1999 and April 2003 who wanted to preserve their fertility via IVF. INTERVE...

Gentle ovarian stimulation protocols, such as 'mini-IVF', have several potential advantages over conventional IVF protocols, including less medication and fewer injections, producing fewer eggs, but eggs of higher quality. The particular 'mild' stimulation protocol called 'mini-IVF' is described. This protocol requires a reliable and cheap method for embryo cryopreservation such as vitrificatio...

17 Introduction 18 Ovarian stimulation 20 In vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) 23 Immature sperm conception 25 Egg donation and surrogacy 28 Oocyte maturation 28 Embryo culture 30 Assisted hatching 31 Cryopreservation 32 Preimplantation genetic diagnosis (PGD) 34 Cytoplasmic donation and mitochondrial DNA (mDNA) transfer 37 Fertility preservation 39 Conclusion 41

The aim of the present pilot study was to assess the feasibility and efficacy of Cetrotide administration in the early luteal phase in patients at high risk of ovarian hyperstimulation syndrome (OHSS), undergoing embryo cryopreservation following superovulation. A total of 135 patients at high risk of OHSS and undergoing embryo cryopreservation were divided into two groups. In the treatment gro...

BACKGROUND Both cleavage-stage and blastocyst-stage embryo transfer policies have advantages and drawbacks. The number of embryos transferred, however, is a crucial parameter that needs to be considered before attempting any comparison. METHODS An extensive literature search yielded initially 282 studies from which 8 randomized controlled trials met the inclusion criteria: (i) truly randomize...

BACKGROUND Embryologists currently face a challenge when counselling patients regarding the stage and the number of embryos to transfer when no top-quality embryos (TQE) are available. METHODS The aim of this study was to evaluate the efficacy of single blastocyst transfer (SBT) in comparison with the transfer of two cleavage-stage embryos in women under 36 years old. A total of 450 women under...