ELISPOT assays are routinely used to measure immune responses of T cells in fresh and frozen splenocytes preparations; however, standardized methods for murine ELISPOT are not widely available. Freezing cells can significantly impact the function of T cells. The aim of this study was to optimize cryopreservation protocols to retain antigen-specific T cell function at similar levels as freshly i...

Study Background: Previously, we demonstrated safety and correlated induced immune response with survival in a Phase I study of FANG immunotherapy in advanced cancer patients. We now report long term follow-up (FU) of Phase I treated patients including assessment of relationships of dose, γIFN-ELISPOT response, and patient demographics to safety and survival. Methods: Safety, γIFN-ELISPOT respo...

10 JALA Febru The Elispot technique has gained much attention over the past few years. Its outstanding sensitivity and general ease of performance has made it one of the most attractive assay candidates for immunomonitoring purposes, including vaccine development and vaccine trials in various phases, diagnostics, and basic research. The demands of the market call for a simplified and automated ...

T lymphocytes play positive and negative roles in the pathogenesis of allergic disease. Isolation and functional characterization of T lymphocyte subpopulations is an important aspect of understanding allergy models and allergy therapies. Measurement of the T cell surface proteins and T cell proliferation can provide insight into T cell activation. T cell function and the identities of T cell s...

Central nervous system tuberculosis remains a clinical diagnostic challenge. The ex vivo Mycobacterium tuberculosis-specific enzyme-linked immunospot assay (ELISPOT) is a novel assay for the rapid detection of M. tuberculosis-specific T-lymphocytes in the peripheral blood. However, when performed on peripheral blood, this assay cannot distinguish between active tuberculosis or latent tuberculos...

BK virus (BKV) infection after kidney transplantation can cause BKV nephropathy (BKVAN) resulting in graft dysfunction and allograft loss. The treatment for BKVAN is reduction of the immunosuppressive load which increases risk transplant rejection. There no biomarker to monitor activity besides viral load. value Enzyme-Linked Immunosorbent Spot (ELISPOT) assay as a tool recipient's anti-BKV imm...

Constant antigenic drift of circulating influenza viruses leads to inefficiency seasonal vaccines, thus requiring annual re-design these vaccines. Therefore, the development a universal vaccine is particular relevance. A promising line research in this area design immunogens consisting conserved protein fragments from different viral strains. The aim work was assess immunogenicity DNA vaccines ...

Table S2. HLA allele information of ELISpot assay responders and its compatibility with previous report. In total, 79 responses among 14 epitopes were identified. HLA allele information of ELISpot assay responders and its compatibility with previous report of responsible HLA alleles listed in Los Alamos database are shown. Copyright: ß 2014 The PLOS ONE Staff. This is an open-access article dis...

Results There were a total of 24 patients. The prevalence of LTBI at inclusion was 20.8% (5/24 patients). The incidence of LTBI after initiation of immunosuppression was 26.3% (5/19 patients) and the prevalence of LTBI in the study as a whole was 41.6% (10/24 patients). The presence of epidemiological history positive for TB showed a relative risk of 2.0 for the development of LTBI. No patient ...