Introduction: Helicobacter pylori, as an etiologic cause of peptic ulcers and gastric cancer, should be tested and treated. The true state of infection can only be detected by isolation of gastric biopsies through the invasive method of gastroscopy. However, there are several non-invasive methods for detection of infection, the most common of which is serology. Methods: Here we have evaluated t...

BACKGROUND Anti-ribosomal-P antibodies have been associated with central nervous system manifestations of systemic lupus erythematosus. However, inconsistencies in their prevalence and clinical correlations have become an obstacle to their use as a diagnostic marker of the disease. This lack of consistency might stem from several factors, such as the lag period between clinical manifestations a...

BACKGROUND Bovine viral diarrhea (BVD) is an economically important disease of cattle distributed worldwide. Diagnosis of BVD relies on laboratory-based detection of its viral causing agent or virus specific antibodies and the most common laboratory method for this purpose is Enzyme-Linked Immunosorbent Assay (ELISA). OBJECTIVES The current study was aimed to develop a simple indirect ELISA t...

Hepatitis B virus (HBV) DNA diagnosis plays a critical role in the transmission of HBV infections. Accurate protocols detection will reduce spread from blood donors. Therefore, this research compared detecting potentials three different detection. Blood 200 donors at University college bank Ibadan were sampled and tested for using ABON™ HBsAg Surface Antigen Tests (strip test), Monolisa TM ULTR...

Hepatitis B virus (HBV) infection is of global public health concern. Among various serological tests used for the diagnosis and screening of HBV infection, the enzyme-linked immunosorbent assay (ELISA) to detect hepatitis B surface antigen (HbsAg) is most widely used. The present study was designed to develop and standardize a cost effective in-house ELISA for the detection of HbsAg and compar...

For evaluating the antigen content in recombinant Hepatitis B Vaccine (Monovalent and Pentavalent vaccines), National Control Laboratory, Kasauli has taken an initiative to develop a uniform method for evaluation of antigen content of Hepatitis B vaccine. All the manufacturers in India are having different method for evaluating Relative Potency of Hepatitis B Vaccine. In the present study the s...

BACKGROUND Without appropriate culture systems for hepatitis E virus (HEV), sufficient natural viral proteins are difficult to generate for use in serological tests. Therefore, it is important to produce large amounts of HEV recombinant proteins in an economical way. The present study developed ELISAs using 2 truncated forms of the HEV open reading frame (ORF) 2 protein in order to detect anti-...

was quantified by integration using EZLogicTM integration software (Bio-Rad). The clearance of host cell proteins (HCP) and double-stranded DNAs (dsDNA) was determined by a CHO-CM HCP ELISA kit (Cygnus Technologies) and Quant-iT dsDNA high-sensitivity assay kit (Life Technologies Corporation), respectively. mAb1 concentration was determined by UV absorption at 280 nm using a coefficient of 1.4 ...