Pacific herring Clupea pallasi immunoglobulin is an IgM-like molecule comprised of heavy and light chains with molecular weights of 79 and 25 to 27 kD, respectively. Purified immunoglobulin was used to generate highly specific polyclonal antibodies for development of a sandwich ELISA. The ELISA was used to quantify total plasma IgM in 602 Pacific herring captured in Prince William Sound and Sit...

In this study, we compared Coxiella burnetii IgG phase I, IgG phase II, and IgM phase II detection among a commercially available enzyme-linked immunosorbent assay (ELISA) (Virion/Serion), an indirect fluorescent antibody test (IFAT) (Focus Diagnostics), and a complement fixation test (CFT) (Virion/Serion). For this, we used a unique collection of acute- and convalescent-phase sera from 126 pat...

The PanBio Leptospira immunoglobulin M (IgM) enzyme-linked immunosorbent assay (ELISA) is a commercially available screening test for the diagnosis of acute leptospiral infection. The ability of the test to diagnose early or recent Leptospira interrogans infection was assessed by testing sera with known microagglutination test (MAT) titers to serovars pomona, hardjo, copenhageni, and australis....

Based on algorithms of hidrophilicity and flexibility, obtained by the use of the ProtScale software, disposable at the site http:// au.expasy.org/cgi-bin/protscale.pl, seven potentially antigenic peptides were selected from different Schistosoma mansoni proteins: cathepsin B (Sm31), heat shock protein (SmHSP-70), cathodic circulating antigen (CCA) and the polypeptidic sequence of the open read...

The outer surface protein C (OspC) and the internal 14-kDa flagellin fragment of strain GeHo of Borrelia burgdorferi sensu stricto were expressed as recombinant proteins in Escherichia coli and were purified for use in an immunoglobulin M (IgM) enzyme-linked immunosorbent assay (OspC-14-kDa antigen ELISA). No hint at disturbing protein-protein interferences, which might influence the availabili...

The enzyme-linked immunosorbent assay (ELISA) was applied for immunological diagnosis of human tularemia, using lipopolysaccharide from Francisella tularensis as antigen. Sera collected from patients, healthy individuals, and vaccinated volunteers were investigated for antibodies against F. tularensis by ELISA and tube agglutination. In ELISA all sera were titrated with a polyspecific anti-immu...

Objetivo. Determinar la circulación del virus Oeste Nilo (VON) en caballos oriente de Yucatán, México. Materiales y métodos. Por conveniencia se estudiaron 184 localizados 23 unidades producción los municipios Tizimín Panabá, Yucatán. De cada caballo obtuvo una muestra suero sanguíneo, cual fue evaluada con un ensayo inmunoabsorción enzimática captura (MAC-ELISA) para detectar inmunoglobulinas ...

Abstract Hemophilia A is an X-linked bleeding disorder resulting in events due to insufficient levels of factor VIII (FVIII). The most significant complication the management hemophilia formation polyclonal neutralizing IgG (i.e., inhibitors) which impede FVIII procoagulant activity. In a (FVIII KO) murine model injected with multiple doses FVIII, FVIII-specific IgM were found persist despite e...

Leptospirosis is a serious zoonosis that is underdiagnosed because of limited access to laboratory facilities in Southeast Asia, Central and South America, and Oceania. Timely diagnosis of locally distributed serovars of high virulence is crucial for successful care and outbreak management. Using pooled patient sera, an expression gene library of a virulent Leptospira interrogans serovar Autumn...