نتایج جستجو برای: electroporation
تعداد نتایج: 4856 فیلتر نتایج به سال:
The movement of ions through the cell membrane is in the literature and in textbooks explained by the action of protein ion channels. An exception is the field of electroporation. Electroporation is a temporary reversible electrical breakdown induced by lipid channels in the membrane. The lipid channels are caused by large trans membrane voltages, hence the pore models of electroporation are no...
BACKGROUND Radiofrequency ablation inside pulmonary vein (PV) ostia can cause PV stenosis. A novel alternative method of ablation is irreversible electroporation, but the long-term response of PVs to electroporation ablation is unknown. METHODS AND RESULTS In ten 6-month-old pigs (60-75 kg), the response of PVs to circular electroporation and radiofrequency ablation was compared. Ten consecut...
Electroporation is commonly used to deliver molecules such as drugs, proteins, and/or DNA into cells, but the mechanism remains poorly understood. In this work a rapid microfluidic assay was developed to determine the critical electric field threshold required for inducing bacterial electroporation. The microfluidic device was designed to have a bilaterally converging channel to amplify the ele...
Chicken embryonic retina is an excellent tool to study retinal development in higher vertebrates. Because of large size and external development, it is comparatively very easy to manipulate the chick embryonic retina using recombinant DNA/RNA technology. Electroporation of DNA/RNA constructs into the embryonic retina have a great advantage to study gene regulation in retinal stem/progenitor cel...
AIM OF THE STUDY Irreversible electroporation is a new, non-thermal ablation technique in the treatment of parenchymal organ tumors which uses short high voltage pulses of electricity in order to induce apoptosis of targeted cells. In this paper the application of this method of treatment in locally advanced pancreatic cancer (LAPC) and liver cancer is analyzed. MATERIAL AND METHODS Between 0...
There is limited information using irreversible electroporation to treat pancreatic tumors. We report a case of a 78-year-old male who underwent irreversible electroporation of a pancreatic neuroendocrine tumor.
Plasmodium falciparum malaria is one of the leading infectious causes of childhood mortality in Africa. EP-1300 is a polyepitope plasmid DNA vaccine expressing 38 cytotoxic T cell epitopes and 16 helper T cell epitopes derived from P. falciparum antigens expressed predominantly in the liver phase of the parasite's life cycle. We performed a phase 1 randomized, placebo-controlled, dose escalatio...
The zebrafish has various advantages as a model organism to analyze the structure and function of neural circuits but efficient viruses or other tools for fast gene transfer are lacking. We show that transgenes can be introduced directly into the adult zebrafish brain by herpes simplex type I viruses (HSV-1) or electroporation. We developed a new procedure to target electroporation to defined b...
Single-cell electroporation allows the transfection of a small number of neurons in an organotypic culture with a single plasmid or a defined mixture of plasmids. Desired protein expression levels can vary depending on the experimental goals (e.g., high expression levels are needed for optogenetic experiments); however, when too much protein is expressed, cellular toxicity and cell death may ar...
In ovo electroporation is an excellent method to ectopically induce or inhibit gene expression in chicken embryos and to study the in vivo function of genes during embryonic development. However, the application of electroporation in ovo to date is limited to an early stage of incubation (< stage 20). In older embryos (> stage 22), the vitelline and allantoic vessels have developed extensively ...
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