tau.amegroups.com © Translational Andrology and Urology. All rights reserved. Dr. Ahmad, in his well-written commentary (1), shared his viewpoints about the utility of sperm DNA fragmentation (SDF) testing on each clinical scenario proposed in the practice recommendations by Agarwal et al. (2). He highlighted the pitfalls of relying on quantitative semen parameters in current practice and the n...

OBJECTIVE To examine the presence of cleaved poly(ADP-ribose) polymerase(s) (cPARP) in ejaculated spermatozoa and determine cPARP levels following exposure to chemical or oxidative stress. DESIGN Prospective pilot study. SETTING Tertiary care academic hospital. PATIENT(S) Eight healthy men. INTERVENTION(S) Semen specimens were collected, prepared with double-density-gradient centrifugat...

Koonjaenak S. 2006. Semen and Sperm Characteristics of Swamp Buffalo (Bubalus bubalis) Bulls for Artificial Insemination in Thailand, in Relation to Season. Doctoral thesis. ISSN 1652-6880, ISBN 91-576-7263-6 In this thesis work we tested the hypothesis that tropical climatic conditions affect the quality of fresh and frozen-thawed (FT) spermatozoa from swamp buffalo bulls used for artificial i...

Background Estrogens biosynthesis in ejaculated spermatozoa is an autonomous process that may influence sperm functions. The purpose of this study was to evaluate the relationship between the expression of aromatase, sperm quality and seminal neutral α–glucosidase marker in semen of Tunisian infertile men. MaterialsAndMethods Fifty seven men were investigated: they were composed of two groups: ...

Controversial reports have been published about the influence of sperm source and of the underlying testicular pathology on success rates of intracytoplasmic sperm injection (ICSI). In this controlled study, ICSI treatment cycles with testicular spermatozoa from men with obstructive and non-obstructive azoospermia were compared with ICSI ejaculated sperm cycles with semen parameters < or = 5 x ...

The invasion of the male reproductive tract by microorganisms, and its subsequent consequences for sperm fertilizing potential, has been intensely discussed. The role of the bacteria that are responsible for the colonization and contamination of the male urogenital tract, rather than its infection, in diminished sperm parameters raises the most controversy. There are numerous premises suggestin...

Natural reproduction is an interaction process between gametes, female (oocyte) and male (spermatozoon), that occurs after spermatozoon travel along reproductive sexual tract during which it suffers several physiological and biochemical changes called capacitation. After capacitation, the spermatozoa are capable of undergoing acrosome reaction upon stimulation by the pellucid zone. In humans du...

The aim of this study is to underline the role of transmission and scanning electron microscopy analysis in the diagnosis of human sperm pathologies. Among the possible causes of male infertility, defects of sperm morphology represent an important factor that may explain decreased fertilizing potential of sperm. Electron microscopy allowed us to identify systematic sperm defects that affect the...