The resistance gene for beta-lactamase-stable cephalosporins from Enterobacter cloacae was transferred to Escherichia coli by the aid of RP4::mini-Mu. The R-prime plasmids generated carried 60 to 80 kilobases (kb) of E. cloacae DNA and coded for the chromosomal E. cloacae beta-lactamase. The gene was fully expressed in the recipient. Restriction endonuclease EcoRI fragments of the R-prime plasm...

Many enteric bacteria express a type I oxygen-insensitive nitroreductase, which reduces nitro groups on many different nitroaromatic compounds under aerobic conditions. Enzymatic reduction of nitramines was also documented in enteric bacteria under anaerobic conditions. This study indicates that nitramine reduction in enteric bacteria is carried out by the type I, or oxygen-insensitive nitrored...

OBJECTIVE The present study was designed to evaluate the molecular epidemiology of CTX-M producing Klebsiella pneumoniae, Enterobacter cloacae and Escherichia coli isolated from bloodstream infections at tertiary care hospitals in the State of Rio de Janeiro, Brazil. MATERIAL AND METHODS A total of 231 nonduplicate Enterobacteriaceae were isolated from five Brazilian hospitals between Septemb...

MICs of DU-6859a, a novel fluoroquinolone, for 18 Klebsiella pneumoniae isolates and 21 Enterobacter cloacae isolates with altered GyrA or altered GyrA and ParC ranged from < or =0.025 to 6.25 microg/ml and from 0.1 to 3.13 microg/ml, respectively. Based on the MICs at which 90% of the isolates were inhibited for these strains of K. pneumoniae and E. cloacae, DU-6859a exhibited 16- to 256-fold-...

Technical Appendix Table. β-lactamase profiles of multidrug-resistant Gram-negative bacilli isolated in 2010 and 2011, France* Region of origin No. culture-positive patients/total no. patients Species No. isolates β-lactamase ESBL Carbapenemase pCASE Africa 33/87 E. coli 18 CTX-M-15 2 CTX-M-1 2 SHV-12 1 CTX-M-3 OXA-48 1 CTX-M-14 1 CTX-M-15 TEM-169 1 CTX-M-15 CMY-2 1 CTX-M-24 OXA-48 1 ND K. pneu...

There is an error in the penultimate sentence of the “Detection of blaNDM-1 positive isolates” subsection of the Results. The correct sentence is: These isolates were collected from 8 individual patients, consisting of 5 male (62.5%) and 3 female (37.5%) with a mean age of 29.7 years, including 2 infants (ECL-2, ECL-36). There is an error in Table 2, “Characteristics of blaNDM-1-positive E. clo...

Susceptibility to piperacillin was similar to that to piperacillin-tazobactam (<1% difference) for 6,938 isolates of Enterobacter aerogenes and 13,954 isolates of Enterobacter cloacae tested using a Vitek system; for the same species, in contrast, susceptibility rates to piperacillin-tazobactam were 5.9 to 13.9% higher than to piperacillin using disk diffusion, MicroScan, and Vitek 2 testing. U...

* Corresponding author. E-mail: [email protected] The Escherichia coli asr gene and its orthologues in the genomes of other members of the family Enterobacteriaceae represent stress response elements regulated by a low environmental acidity (pH < 5.0) [1]. They code for small basic 82–139 amino acid proteins of unknown function. While important for exponential phase E. coli growth in ...

Enterobacter aerogenes and E. cloacae have been reported as important opportunistic and multiresistant bacterial pathogens for humans during the last three decades in hospital wards. These Gram-negative bacteria have been largely described during several outbreaks of hospital-acquired infections in Europe and particularly in France. The dissemination of Enterobacter sp. is associated with the p...

Background. Preventing the spread of carbapenem-resistant Enterobacteriaceae (CRE) is important to public health because of high infection-related morbidity, mortality, and cost. Carbapenemase producing (CP)-CRE are most concerning because of their rapid global dissemination. In 2013, CRE in Oregon were defined as Enterobacteriaceae with non-susceptibility to ≥1 carbapenem, including ertapenem,...