The analysis of environmental DNA (eDNA) by high throughput sequencing (HTS) is proving to be a promising tool for freshwater fish biodiversity assessment in Europe within the Water Framework Directive (WFD, 2000/60/EC), especially large rivers and lakes where current monitoring techniques have known shortcomings. These new biomonitoring methods based on eDNA show several advantages compared cl...

thymus is a medicinal plant which contains one of the world's top ten essential oils, exhibiting antibacterial, antioxidative, food preservative and mammalian age-delaying properties. this work was aimed at identifying between-species variations requiring for selecting appropriate parents for hybridization. six iranian endemic thymus accessions belonging to thymus daënensis, t. eriocalyx and t....

The techniques of molecular biology have had a dramatic effect on the advancement of human genetics. In particular, the development of restriction fragment length polymorphisms (RFLPs) has allowed researchers to generate genetic markers for virtually any region of the human genome. Most RFLPs occur when a mutation creates or deletes a recognition site for a restriction enzyme, generating a DNA ...

objectives: small dense low‑density lipoprotein (sdldl) which has a small ldl particle size with greater susceptibility to oxidation is regarded as a risk marker for cardiovascular disease. the diacron reactive oxygen metabolites (d‑roms) test has recently been introduced as an oxidative stress‑related marker in the clinic. the aim of the present study was to investigate the correlation between...

A polymorphic DNA marker has been found genetically linked, in a set of 39 human families, to an autosomal recessive gene that causes cystic fibrosis (CF), a disease affecting one in 2000 Caucasian children. The DNA marker (called D0CRI-917) is also linked to the PON locus, which by independent evidence is linked to the CF locus. The best estimates of the genetic distances are 5 centimorgans be...

InDel (insertion deletion length polymorphism) markers is designed for PCR detection based on the Insertion or of DNA fragments different sizes according to sequence a locus between individuals related species, which more and applied in crop genetic research due its rich polymorphism, operational simplicity reliability results. In this study, Qinyou 88 parents were analyzed through marker, four...

Purified DNA from three different types of mammalian cells was precipitated with calcium phosphate and added to mouse L cells deficient in thymidine kinase (TK). Donor DNA was prepared from three cell lines: (a) mouse cells transfected with UV-inactivated herpes simplex virus (HSV) type 1, or a purified fragment of HSV carrying the TK gene (b) human HeLa cells, and (c( CHO, a cell line derived ...