Polymerase chain reaction (PCR) is a powerful tool with many different applications in Molecular Biology and related disciplines. Amplification of the inserts cloned into a vector directly from the bacterial colony is one of them. This technique allows a fast screening of many different clones in order to detect which one of them contains the desired construction. The primers used to direct thi...

Genetic variation and inheritance of randomly amplified polymorphic DNA (RAPD) markers in female grass carp (Ctenopharyngodon idella) and male bighead carp (Hypophthalmichthys nobilis) and their F1 offspring hybrid have been studied. For this purpose, genomic DNA was extracted from muscle tissue according to phenol-chloroform method, and six decamer primers were used for amplifying polymorphic ...

A polymerase chain reaction (PCR)-based method was developed to detect DNA of Fusarium solani f. sp. glycines , the cause of soybean sudden death syndrome. Two pairs of primers, Fsg1/Fsg2 designed from the mitochondrial small subunit ribosomal RNA gene, and FsgEF1/FsgEF2 designed from the translation elongation factor 1α gene, produced PCR products of 438 and 237 bp, respectively. Primer specif...

Givotia rottleriformis Griff. is an economically important dioecious tree species known for the softwood used in making toys. Knowledge of genetic variation in a dioecious tree species is important for devising strategies for its successful management and conservation. Studies were conducted to examine the genetic variation in Givotia plants using RAPD (random amplified polymorphic DNA) analysi...

background: among the most important parasitic disease, causing diarrhea, gi­ardia lamblia is noteworthy. nowadays detection methods for these parasites in­clude parasitological methods such as microscopic examination. the sensitivity of these methods relies on the expertise and experience of examiners. in contrast, molecular methods such as pcr are less dependent on the expertise of the exam­i...

Information on the genetic structure of fish is a useful means for optimizing identification stocks, stock enhancement, breeding programs, management sustainable yield and preservation diversity. The population African Catfish, Clarias gariepinus (Burchell, 1822) in cultured feral populations from Rivers Guzuguzu, Fada Magaga (Kano State) were investigated using RAPD (Random Amplified Polymorph...

The objective of this research was to establish loop-mediated isothermal amplifications (LAMP) that could be used detect parasitic ciliate Ichthyophthirius multifiliis (I. multifiliis) in freshwater cyprinid fish. Primers were developed from the distinguishing fragments 18S ribosomal RNA I. and LAMP test then evaluate optimize various concentrations chemicals, time temperature. results indicate...