DNA damaging agents are among the most powerful anticancer drugs currently in clinical use. As an alternative to irreversible nucleobase damage and DNA strand breaks, the non-covalent stabilization of unusual, non-B DNA structures is currently emerging as a promising way to cause DNA damage with a high level of specificity. One of such non-B DNA structures is the three-way DNA junction: this Y-...

Nucleobase Orientation and Ordering in Films of Single-Stranded DNA on Gold Dmitri Y. Petrovykh,* V. Pérez-Dieste, Aric Opdahl, Hiromi Kimura-Suda, J. M. Sullivan, Michael J. Tarlov, F. J. Himpsel, Lloyd J. Whitman Physics Department, University of Maryland, College Park, MD 20742; Naval Research Laboratory, Washington, DC 20375; Physics Department, University of Wisconsin, Madison, WI 53706; N...

Two novel modified 2'-deoxyuridine triphosphates carrying an azide functionality linked to the nucleobase were synthesized. For probing the sterical influence on enzymatic incorporation and Staudinger ligation, differently sized flexible linkers were chosen. Both nucleotides can completely replace natural thymidine in primer extension as well as polymerase chain reaction (PCR) using Pyrococcus ...

Nucleobase and nucleoside compounds exist widely in various organisms. An often occurring problem in the discovery of new bioactive compounds from natural products is reisolation of known nucleobase and nucleoside compounds. To resolve this problem, a capillary electrophoresis-high resolution mass spectrometry (CE-HR-MS) method providing both rapid separation and accurate mass full-scan MS data...

When 2-aminopurine (2AP) is substituted for adenine in DNA, it is widely accepted that its fluorescence spectrum is essentially unchanged from that of the free fluorophore. We show that 2AP in DNA exhibits long-wavelength emission and excitation bands, in addition to the familiar short-wavelength spectra, as a result of formation of a ground-state heterodimer with an adjacent, pi-stacked, natur...

The interaction of DNA nucleobases with monolayer GaSe has been studied in DFT framework using vdW functional. We found that are physisorbed on the monolayer. order binding energy per atom is C > T G A. room temperature recovery time estimated to be maximum 113.88 µs for + indicting reusability based devices. modulation electronic structures clearly captured within simulated STM measurements. a...

The reactivity of two DNA-targeted platinum-acridine conjugates with cysteine sulfur was studied. The conjugate containing an amidine-NH donor group cis to the chloride leaving group showed considerably reduced reactivity with N-acetylcysteine compared to the prototypical derivative containing a thiourea-S linkage. The opposite scenario has been observed previously in reactions with nucleobase ...