Herein we describe the first application of direct linear analysis (DLA) to the mapping of a bacterial artificial chromosome (BAC), specifically the 185.1 kb-long BAC 12M9. DLA is a single molecule mapping technology, based on microfluidic elongation and interrogation of individual DNA molecules, sequence-specifically tagged with bisPNAs. A DNA map with S/N ratio sufficiently high to detect all...

We report the discovery and analysis of the most metal-poor damped Lyman α (DLA) system currently known, based on observations made with the Keck HIRES spectrograph. The metal paucity of this system has only permitted the determination of three element abundances: [C/H]=−3.43± 0.06, [O/H]=−3.05± 0.05 and [Si/H]=−3.21± 0.05, as well as an upper limit on the abundance of iron: [Fe/H] ≤ −2.81. Thi...

BACKGROUND The aim of the present study is to analyse the factors associated with mortality and the capacity to perform daily life activities (DLA) in patients with surgically treated proximal humeral fractures. METHODS A retrospective study was conducted on 94 patients with a surgically treated proximal humeral fracture, with a mean follow-up of 8 years (2 - 12 years). A correlation analysis...

BACKGROUND Laser therapy in patients with skin of color is associated with an increased rate of complications. The 755-nm picosecond laser with the diffractive lens array (DLA) has been used for the treatment of scars, striae, and rejuvenation. By delivering high energy to focused areas, the DLA minimizes complications. OBJECTIVE This study explores the adverse events associated with treatmen...

We examine the gas kinematics of the LMC revealed by the high spatial and velocity resolution H I data cube of the combined ATCA and Parkes telescope surveys. We adopt an approach designed to facilitate comparisons with quasar absorption line observations, in particular restricting our analysis to pointings with H I column density satisfying the damped Lyα (DLA) criterion. We measure velocity w...

This study was designed to isolate the compounds responsible for the cytotoxic properties of South Indian Elephantopus scaber L. and further investigate their effects on quiescent and proliferating cells. Bioassay-guided isolation of the whole plant of chloroform extract of South Indian Elephantopus scaber afforded the known sesquiterpene lactone, deoxyelephantopin, and isodeoxyelephantopin who...

A high enantiomer-selectivity for the polymerization of rac-lactide was achieved using chiral binaphthol-derived monophosphoric acids as organocatalysts. During the polymerization, d-lactide (DLA) preferentially polymerized via kinetic resolution with the maximum selectivity factor (kD/kL) of 28.3. The selective polymerization of DLA was derived from a dual activation, i.e., monomer activation ...

Coverage has been a constant thorn in the side of deployed deep linguistic processing applications, largely because of the difficulty in constructing, maintaining and domaintuning the complex lexicons that they rely on. This paper reviews various strands of research on deep lexical acquisition (DLA), i.e. the (semi-)automatic creation of linguistically-rich language resources, particularly from...

Observations of damped Lyα absorbers (DLA) indicate that the fraction of hydrogen in its neutral form (HI) is significant by mass at all redshifts. This gas represents the reservoir of material that is available for star formation at late times. As a result, observational identification of the systems in which this neutral hydrogen resides is an important missing ingredient in models of galaxy ...

This study intended to examine the effect of 3,4-dihydroxy-phenyl lactic acid (DLA), a major ingredient of Salvia miltiorrhiza, on ischemia-reperfusion (I/R)-induced rat mesenteric microcirculatory injury. DLA (5 mg.kg(-1).h(-1)), superoxide dismutase (SOD, 12,000 U.kg(-1).h(-1)), or catalase (CAT, 20 mg/kg) was continuously infused either starting from 10 min before the ischemia or 10 min afte...