We developed and characterized replicative small interfering RNA (siRNA) vectors for efficient, specific, and long-term gene silencing in human cells. We created stable XPA(KD) and XPC(KD) (knockdown) syngeneic cell lines to mimic human cancer-prone syndromes. We also silenced (HSA)KIN17. Several clones displaying undetectable protein levels of XPA, XPC, or (HSA)kin17 were grown for more than 3...

This paper discusses the role and integration of knowledge discovery (KD) in case-based reasoning (CBR) systems. The general view is that KD is complementary to the task of knowledge retaining and it can be treated as a separate process outside the traditional CBR cycle. Unlike knowledge retaining that is mostly related to casespecific experience, KD aims at the elicitation of new knowledge tha...

A sex steroid-binding protein (SBP) that binds to 17 b-estradiol with high affinity and moderate capacity was identified in the plasma of female and juvenile of kutum (Rutilus frisii kutum) sampled during the early stage of gonadal maturation in October and prior to spawning in March. Mean maximum specific binding (Bmax) and equilibrium dissociation constant (Kd) of the fish were as follows: In...

background: the ketogenic diet (kd), high in fat and low in carbohydrate and protein, provides sufficient protein but insufficient carbohydrates for all the metabolic needs of the body. kd has been known as a therapeutic manner intractable epilepsy. in recent years, the effectiveness of kd drew attention to the treatment of some other disorders such as parkinson’s disease (pd). this study has e...

A sex steroid-binding protein (SBP) that binds to 17 b-estradiol with high affinity and moderate capacity was identified in the plasma of female and juvenile of kutum (Rutilus frisii kutum) sampled during the early stage of gonadal maturation in October and prior to spawning in March. Mean maximum specific binding (Bmax) and equilibrium dissociation constant (Kd) of the fish were as follows: In...

kawasaki disease (kd) is an acute febrile systemic vasculitis of childhood characterized by elevated levels of inflammatory mediators at the acute stage. high-dose intravenous immunoglobulin (ivig) is well accepted as a conventional therapy for kd. the aim of the present study was to determine the expression level of toll like receptors (tlrs) and their corresponding signaling mediators in pbmc...

A novel Schiff base-chitosan-grafted multiwalled carbon nanotubes (S-CS-MWCNTs) solid-phase extraction adsorbent was synthesized by covalently grafting a Schiff base-chitosan (S-CS) onto the surfaces of oxidized MWCNTs. The stability of a chemically(S-CS-MWCNTs) especially in concentrated hydrochloric acid which was then used as a recycling and pre-concentration reagent for further uses of (S-C...

BACKGROUND AND PURPOSE The significance and physiological implications of the expression of the 72-kd heat-shock protein in ischemic tissue are unknown. To enhance our understanding of the relation between ischemic cell damage and 72-kd heat-shock protein expression, we evaluated the cellular expression and the anatomic distribution of 72-kd heat-shock protein in conjunction with the morphologi...

In analysis of problems with parametric spline boundaries that are immersed or inserted into an underlying domain, the discretization on domain usually does not conform to boundaries. While fixed is great convenience as evolve, field approximations near require refinement in do quadrature cells. this paper, a kd-tree data structure together sign-based and/or distance-based strategy proposed for...