Abstract There is increasing attention to the production of cellulose nanocrystals (CNCs) from lignocellulosic biomass by enzymatic hydrolysis with cellulase. In this study, feasibility application a cellulase system engineered strain Penicillium oxalicum cEES in CNCs was assessed. Using commercial eucalyptus dissolving pulp (EDP) as substrate, were successfully obtained cEES, and total yields ...