Disassembly is one of the most time-consuming and labor-intensive activities during value recovery end-of-life (EOL) products. The completion time (makespan) disassembling EOL products highly associated with allocation operators, especially in parallel disassembly. In this paper, asynchronous disassembly planning (APDP), which avoids necessity to synchronize tasks manipulators process, studied ...

The nuclear lamina lines the inner nuclear membrane providing a structural framework for the nucleus. Cellular processes, such as nuclear envelope breakdown during mitosis or nuclear export of large ribonucleoprotein complexes, are functionally linked to the disassembly of the nuclear lamina. In general, lamina disassembly is mediated by phosphorylation, but the precise molecular mechanism is s...

Molecular markers of the zebrafish inner nuclear membrane (NEP55) and nuclear lamina (L68) were identified, partially characterized and used to demonstrate that disassembly of the zebrafish nuclear envelope requires sequential phosphorylation events by first PKC, then Cdc2 kinase. NEP55 and L68 are immunologically and functionally related to human LAP2beta and lamin B, respectively. Exposure of...

Using sea urchin embryonic and in-vitro-assembled nuclei incubated in sea urchin mitotic extract, I provide evidence for a requirement for functional nuclear pores and a nuclear lamina for nuclear envelope disassembly in vitro. In interphase gastrula nuclei, lamin B interacts with p56, an integral protein of inner nuclear membrane cross-reacting with antibodies to human lamin B receptor. Incuba...

The primary cilium is a non-motile microtubule-based organelle that protrudes from the surface of most human cells and works as a cellular antenna to accept extracellular signals. Primary cilia assemble from the basal body during the resting stage (G0 phase) and simultaneously disassemble with cell cycle re-entry. Defective control of assembly or disassembly causes diverse human diseases includ...

Many proteins are known to promote ciliogenesis, but mechanisms that promote primary cilia disassembly before mitosis are largely unknown. Here we identify a mechanism that favours cilium disassembly and maintains the disassembled state. We show that co-localization of the S/G2 phase kinase, Nek2 and Kif24 triggers Kif24 phosphorylation, inhibiting cilia formation. We show that Kif24, a microtu...

Myelin is essential in vertebrates for the rapid propagation of action potentials, but the molecular mechanisms driving its formation remain largely unknown. Here we show that the initial stage of process extension and axon ensheathment by oligodendrocytes requires dynamic actin filament assembly by the Arp2/3 complex. Unexpectedly, subsequent myelin wrapping coincides with the upregulation of ...

In this paper we consider the problem of designing a mixed assembly–disassembly line for remanufacturing. That is, parts from the disassembly and repair of used products can be used to build “new” products. This is a problem common to many OEM remanufacturers, such as Xerox or Kodak. We study two main configurations, under the assumption that the disassembly sequence is exactly the reverse of t...

The objective of the Disassembly Line Balancing Problem (DLBP) is to use the resources of the disassembly line as efficiently as possible while meeting the demand. This issue is hard to attempt due to the inherent uncertainties that occur during the process. Starting from real industrial examples, this article presents a simple-to-apply method to accomplish the balancing of complex disassembly ...