Turnover of actin networks in cells requires the fast disassembly of aging actin structures. While ADF/cofilin and Aip1 have been identified as central players, how their activities are modulated by the architecture of the networks remains unknown. Using our ability to reconstitute a diverse array of cellular actin organizations, we found that ADF/cofilin binding and ADF/cofilin-mediated disass...

Heubes, S. and Stemmann, O. (2007). The AAA-ATPase p97-Ufd1-Npl4 is required for ERAD but not for spindle disassembly in Xenopus egg extracts. Securin is not required for chromosomal stability in human cells. PLoS Biol 3, e416. Contents CONTENTS 1 SUMMARY 1 2 INTRODUCTION 3 2.1 The eukaryotic cell cycle – An overview 3 2.1.1 Establishment and resolution of cohesion between sister chromatids 4 2...

Disassembly is not only a premise of products recycling, but also an important link of products remanufacturing. However, used products suffer from the influence of a variety of uncertainties. The randomness of disassembly process is a significant feature. In this paper, a disassembly network is established, in which lengths of arc are stochastic variables with a specified power subject to spec...

On linear single-stranded DNA, RecA filaments assemble and disassemble in the 5' to 3' direction. Monomers (or other units) associate at one end and dissociate from the other. ATP hydrolysis occurs throughout the filament. Dissociation can result when ATP is hydrolyzed by the monomer at the disassembly end. We have developed a comprehensive model for the end-dependent filament disassembly proce...

Cloud based approach for remanufacturing is becoming a new technical solution for sustainable management of Waste Electrical and Electronic Equipment (WEEE). This paper presents a service-oriented framework of a Cloud Based Remanufacturing System (CBRS) for WEEE. In remanufacturing of WEEE, disassembly plays an important role. However, complete disassembly is rarely an ideal solution due to the...

Disassembly of RecA protein subunits from a RecA filament has long been known to occur during DNA strand exchange, although its importance to this process has been controversial. An Escherichia coli RecA E38K/DeltaC17 double mutant protein displays a unique and pH-dependent mutational separation of DNA pairing and extended DNA strand exchange. Single strand DNA-dependent ATP hydrolysis is catal...

BACKGROUND Many of the diverse functions of cilia depend upon tight control of their length. Steady-state length reflects a balance between rates of ciliary assembly and disassembly, two parameters likely controlled by a length sensor of unknown identity or mechanism. RESULTS A null mutation in Chlamydomonas CNK2, a member of the evolutionarily conserved family of NIMA-related kinases, reveal...

We describe a cell-free system in which a postribosomal supernatant (s140) from metaphase Chinese hamster ovary (CHO) cells induces prophase-like changes in isolated CHO cell nuclei, including chromatin condensation, and nuclear envelope and lamina disassembly. These events are strongly promoted by gamma-S-ATP and an ATP-regenerating system, and do not take place with an s140 derived from G2-ph...

Disassembly of focal adhesions (FAs) allows cell retraction and integrin detachment from the extracellular matrix, processes critical for cell movement. Growth of microtubules (MTs) can promote FA turnover by serving as tracks to deliver proteins essential for FA disassembly. The molecular nature of this FA "disassembly factor," however, remains elusive. By quantitative proteomics, we identifie...