An efficient protocol for direct shoot organogenesis has been developed for the medicinal plant Aerva lanata (L.) Juss. ex Schult. Regeneration was achieved from leaf segments of 20 days old in vitro plantlets raised on Murashige and Skoog (MS) medium containing 0.25-2.0 mg L(-1) thiadiazuron (TDZ), 3% sucrose, and 0.8% agar. After 21 days of culture incubation, maximum number of shoot organoge...

Atalantia monophylla (L.) DC. is a medium sized medicinal tree used in Ayurveda, Siddha and Traditional systems of medicine. It is used for the treatment of chronic rheumatism and paralysis, as an antidote for snake bites and shows antibacterial, antifungal and antifeedant activity. The study aims to develop a protocol for multiple shoot induction from nodal and root explants. After surface ste...

Potato is one of the most important staple crops in both developed and developing countries of the world. Potato genetic resources are a prerequisite for potato breeding in both conventional and genetic engineering programmes. Cryopreservation has long been considered an ideal means for the long-term conservation of plant genetic resources. This review provides comprehensive information on vari...

An efficient protocol was developed for micro-propagation of Indian papaya (Carica papaya L.) var. Co7. Shoot bud induction occurred on culturing epicotyl segments in MS (Murashige and Skoog, 1962) medium, followed by transfer onto shoot multiplication, elongation and rooting media. Adventitious buds were induced on MS medium supplemented with varying concentration of thidiazuron (TDZ: 0.5-10 μ...

To enhance the genetic resource base of Echinacea purpurea (L.) Moench, a tetraploid was developed by treating seeds with different concentrations of colchicine. For further multiplication, in vitro micropropagation through adventitious shoot induction and proliferation was achieved. Shoot buds were induced directly on adaxial surface of mature leaf tissues growing on MS medium supplemented wit...

The cactus Mammillaria gracillis Pfeiff. was propagated in vitro on Murashige and Skoog (MS) medium without any growth regulators. At the bases of some plants abundant callus masses developed. Lower agar and higher MS salt concentrations stimulated callus pro­ duction. In the culture, morphologically normal and hyperhydric shoots were regenerated. The growth of crown-gall tumour was induced on ...

A highly efficient protocol for in vitro propagation of Rauwolfia serpentina L. was developed that consisted of callus induction, multiple shoot regeneration and rooting stages. Effect of different plant growth regulators, exclusively or in combination on callus induction, multiple shoot formation and root induction, were observed. Maximum callusing (93.65%) were obtained in leaf and stem expla...

Soybean, a major legume crop, is the source of vegetable oil and protein. There is a need for transgenic approaches to breeding superior soybean varieties to meet future climate challenges. Efficient plant regeneration is a prerequisite for successful application of genetic transformation technology. Soybean cultivars are classified into different maturity groups based on photoperiod requiremen...

Leaf and hypocotyl explants of 15 days old aseptically grown seedlings of Solanum laciniatum were cultured on MS medium supplemented with NAA (2 mg/l) and kinetin (0.5 mg/l) for callus initiation. For maintenance and proliferation of callus MS medium supplemented with 2,4-D (1 mg/l) and kinetin (0.5 mg/l) was used. The growth of the calli derived from hypocotyls increased with time of incubatio...

An efficient selection system and Agrobacterium mediated transformation for chickpea (Cicer arietinum L.) cotyledonary nodes were investigated. Effect of selective agents and antibiotics on multiple shoot and root induction of cotyledonary nodes and effects of mechanical injury and vacuum infiltration on transformation efficiency were evaluated. Selective agents and antibiotics were applied to ...