rapidly at room temperature (RT) to provide ready-to-read results within 5 minutes without the need for incubation at elevated temperatures (Figure 1). Similar to the BCA assay, the Pierce Rapid Gold BCA Protein Assay combines the well-known reduction of Cu2+ to Cu+ by proteins in an alkaline medium (biuret reaction) with highly sensitive and selective colorimetric detection by a new copper che...

Introduction: Adherence to the treatment regimen is among the key principles in chronic diseases, like thalassemia. The difficult treatment of these patients and the significant biopsychosocial changes that occur during adolescence may lead to numerous psychosocial changes. Such issuesmay affect their adherence to the treatment regimen. Objective: The current study aimedto deter...

Background:  Deferasirox is an oral iron chelator widely used to treat iron overload in patients with transfusion-dependent β-thalassemia. This study investigated the prevalence of visual and auditory complications caused by deferasirox. Materials and Methods: This cross-sectional study included 156 patients aged less than 18 years with transfusion-dependent β-thalassemia and deferasirox iron...

A phosphonate pendant-armed cross-bridged cyclam chelator has been synthesized, complexed to Cu(ii), radiolabeled with (64)Cu under mild conditions, and its biodistribution studied.

Iron chelation therapy using iron (III) specific chelators such as desferrioxamine (DFO, Desferal), deferasirox (Exjade or ICL-670), and deferiprone (Ferriprox or L1) are the current standard of care for the treatment of iron overload. Although each chelator is capable of promoting some degree of iron excretion, these chelators are also associated with a wide range of well documented toxicities...

Avocado (Persea americana) fruit experience a rapid and extensive loss of firmness during ripening. In this study, we examined whether the chelator solubility and molecular weight of avocado polyuronides paralleled the accumulation of polygalacturonase (PC) activity and loss in fruit firmness. Polyuronides were derived from ethanolic precipitates of avocado mesocarp prepared using a procedure t...

Sickle erythrocytes have increased ferritin and increased molecular iron on the inner membrane leaflet, and we postulated that cytosolic labile iron is also elevated. We used the fluorescent metallosensor, calcein, and a permeant Fe2+ chelator to estimate labile cytoslic Fe2+, and calcein plus an Fe3+ chelator to estimate total cytosolic labile iron (Fe2+ + Fe3+). We measured membrane nonheme i...