Regulation of cell-specific cellulase synthesis (expressed in milligrams of cellulase per gram [dry weight] of cells) by Clostridium thermocellum was investigated using an enzyme-linked immunosorbent assay protocol based on antibody raised against a peptide sequence from the scaffoldin protein of the cellulosome (Zhang and Lynd, Anal. Chem. 75:219-227, 2003). The cellulase synthesis in Avicel-g...

In this paper we propose a novel qualitative formalism to model gene expression dynamics dependent on dilution due to growth rate of the cell. We extend the piecewise linear (PL) systems by keeping the use of step functions to model the interactions between the elements and adding a growth rate expression to model the dilution effect. Focusing on the global gene expression machinery in bacteria...

We demonstrate strong experimental support for the cybernetic model based on maximizing carbon uptake rate in describing the microorganism's regulatory behavior by verifying exacting predictions of steady state multiplicity in a chemostat. Experiments with a feed mixture of glucose and pyruvate show multiple steady state behavior as predicted by the cybernetic model. When multiplicity occurs at...

Abstract Laser Direct Metal Deposition (DMD) has been developed as a manufacturing process to deposit coatings on existing materials and proves advantageous in Additive Manufacturing (AM) of complex precise components. However, it is necessary carefully determine the proper parameter combinations make this method economically viable for industries. The intent study address enhancement productiv...

It is shown that a chemostat with two organisms can be made coexistent by means of feedback control of the dilution rate.

The transient behavior of a continuous culture of Klebsiella pneumoniae with mixed feed of glucose and xylose arising from step-up and step-down in dilution rates and from a feed-switching experiment is presented. he organism gradually switches from simultaneous utilization of the substrates at low growth rates to preferred utilization of the faster substrate (i.e, supporting a higher growth ra...

the present work was aimed to design and develop self-nanoemulsifying drug delivery systems (snedds) with the objective to overcome the problems associated with the delivery of talinolol, a hydrophobic and poorly bioavailable drugs having ph dependant solubility. the solubility of talinolol in various oils, surfactants, cosurfactants and aqueous phases were determined to identify and select the...