We investigated the subcellular distribution of dopamine D(1), D(2) and D(5) receptor subtypes in rat frontal cortex, and examined whether psychostimulant-induced elevation of synaptic dopamine could alter the receptor distribution. Differential detergent solubilization and density gradient centrifugation were used to separate various subcellular fractions, followed by semi-quantitative determi...

Solubilized proteins Detergent-solubilization of membrane proteins has proved to be a successful approach in many cases. Both ionic and nonionic detergents have been used: the choice of detergent may be crucial for retaining protein activity. Generally, detergents should be used at concentrations above their critical micelle concentration (CMC). Micelles formed above the CMC mimic a membrane en...

The ability of various nonionic detergents to solubilize D-alanine carboxypeptidase and other membrane-bound enzymes was correlated to a physical property of the detergent, the HLB number. Only a fairly narrow range of detergents were effective solubilizing agents. Purified carboxypeptidase required either a detergent or detergent plus a lipid fraction for stability. Only those detergents effec...

Obtaining well diffracting crystals of membrane proteins is often challenging, but chances can be improved by crystallizing them in lipidic conditions that mimic their natural environments. One approach the high lipid–detergent (HiLiDe) method, which works mixing target protein with concentrations lipid and detergent prior to crystallization. Although this convenient flexible, understanding eff...

This study was conducted to compare forage quality of two native halophytespecies Camphorosma monspeliaca and Limonium iranicum collected from Meighansaline rangelands near Arak city in center of Iran. Edible biomass of both species wassampled on three phonological stages (vegetative growth, full flowering and seedripening). Forage quality indices such as Crude Protein (CP), Dry Matter Digestib...