background & aim: investigation of suspension cell culture and callus production in catharanthus roseus. catharanthus roseus is an herbaceous perennial plant belongs to the family apocynaceae. c. roseus is important as commercial medicinal plant because of its alkaloidal content. whole plant contains about 130 alkaloids those are classified as vinca alkaloids. vincristine and vinblastine; t...

1. Chloramphenicol has a stimulatory effect on the incorporation of radioactive phosphate into the RNA of perfused rat-liver slices, whole liver homogenates or the liver-cell suspensions, and no effect on the incorporation of [(14)C]adenine and [(14)C]uracil into the RNA of the tissue slices. 2. Chloramphenicol completely inhibits the incorporation of labelled adenine and uracil into the RNA of...

colleagues in JCMM [1]. As noted by the authors, mesenchymal stem cells (MSC) enjoy considerable therapeutic potential in regenerative medicine, cell therapy for select genetic diseases and as immunomodulators [2, 3]. Expanding on a prior study that explored the genetic modification of MSC using HIV-1 derived lentiviral vectors, the authors describe a strategy that minimizes the replicative sen...

Datura innoxia cells from suspension cultures were selected for their ability to grow and divide rapidly in normally lethal concentrations of cadmium. Cells resistant to 12.5, 25, 50, 100, 160, 200, and 250 micromolar cadmium chloride were isolated and utilized to initiate cell suspension cultures resistant to this toxic metal ion. Variant cell lines retained their ability to grow in cadmium af...

hepatomas have demonstrated that a spectrum of such tumors exists with varying degrees of change from the parent hepatocyte (26—30). Such hepatocarcinomas as the Morris 5123 (18) and Reuber H-35 (34), for instance, resemble the parent tissue to a marked degree both morphologically and biochemically (27), and thus fall in the category termed “minimaldeviation hepatomas― by Potter (31). On ...

hepatomas have demonstrated that a spectrum of such tumors exists with varying degrees of change from the parent hepatocyte (26—30). Such hepatocarcinomas as the Morris 5123 (18) and Reuber H-35 (34), for instance, resemble the parent tissue to a marked degree both morphologically and biochemically (27), and thus fall in the category termed “minimaldeviation hepatomas― by Potter (31). On ...

Callus induction and withanolides production through cell suspension culture of Withania coagulans (Stocks) Dunal

Background & Aim: Investigation of suspension cell culture and callus production in Catharanthus roseus. Catharanthus roseus is an herbaceous perennial plant belongs to the family Apocynaceae. C. roseus is important as commercial medicinal plant because of its alkaloidal content. Whole plant contains about 130 alkaloids those are classified as vinca alkaloids...

Many aldehydes are important components of natural flavours. They are used in food, cosmetic, and biomedical industries in large amounts. Plant cells or microorganisms carry out their production by biotransformation, which is one of the biotechnological methods that allow them to be defined as 'natural'. Cell cultures of Silybum marianum and Peganum harmala have been studied with a view to in...