نتایج جستجو برای: depc
تعداد نتایج: 120 فیلتر نتایج به سال:
Many of plant and animal viruses counteract the RNA silencing-mediated defense by encoding diverse RNA silencing suppressors (RSSs). Here, we characterized HVT063, a multifunctional protein encoded by Turkey herpesvirus (HVT), as a silencing suppressor in co-infiltration assays with GFP transgenic Nicotiana benthamiana line 16c. Our results indicated that HVT063 could strongly suppress both loc...
مطالعات سینتیکی و اتصالی آنزیم rnase a در برابر سوبسترای پلیمری rnaو نیز 3-ریبونوکلئوتیدهای آزاد، در محیط بافری تریس edta- (100 میلی مولار تریس؛ ph7/5؛ 2 میلی مولار edta) و در دمای 37 با استفاده از روش طیف نور سنجی افتراقی انجام شده است. منحنی اشباع سنتیکی آنزیم با شکل غیر هیپربولیک خود نمایانگر امکان تبدیل دو ساختار فضائی متفاوت از آنزیم به یکدیگر می باشد که در محدوده بسیار...
The polymerase chain reaction (PCR) is used widely for the in vitro amplification of DNA. The versatility of PCR can be enhanced by the introduction of sequential steps such as nested primer amplifications (1) or re-amplification protocols (2). PCR can also be done on cDNA templates produced by reverse transcription of RNA (3). These protocols require additional opening and closing of the react...
Aims—To determine whether the infiltrating cells in the vitreous and subretinal fluid of patients with proliferative vitreoretinopathy (PVR) express messenger RNA for various cytokines found in this condition. Methods—The presence of mRNA coding for HPRT, IL-6, IL-1â, IL-8, and TNFá was investigated in 20 vitreous and subretinal fluid (SRF) samples from patients with PVR by reverse transcriptas...
The ribonuclease protection assay (RPA) is a commonly used method for the detection and quantitation of specific RNA species from total RNA preparations. This highly sensitive technique allows for the specific detection of target RNA species and is useful for evaluating large numbers of samples. Most standard RPA protocols recommend using 5–20 μg total RNA that is typically purified. However, w...
Laser microdissection (LMD) is a technique that allows the recovery of selected cells and tissues from minute amounts of parenchyma. The dissected cells can be used for a variety of investigations, such as transcriptomic or proteomic studies, DNA assessment or chromosomal analysis. An especially challenging application of LMD is transcriptome analysis, which, due to the lability of RNA, can be ...
Several existing methods to construct cDNA libraries require several thousand cells and involve the lengthy procedure of reverse transcription (RT), restriction, adaptor ligation and vector cloning (5,6), which usually fail to maintain the completeness of a cDNA library, resulting in a loss of rare cDNAs. However, gene expression analysis of specific cell populations within a heterogeneous tiss...
BACKGROUND Plant sterols are the major source of micronutrients and have not shown any obvious side effects in human. β-sitosterol is one of the most prevalent phytosterols which have been recorded in ancient medicinal history for its use in the treatment of many chronic diseases, especially cancer. The modulations of mitogen-activated protein kinases' (MAPKs') play a crucial role in the develo...
The analysis of the cellular and molecular metabolism of anticancer drugs has provided important information for optimizing the design of clinical trials (9). In recent years, there has been an increasing use and development of RNAdirected chemotherapeutic agents, such as ribonucleotide analogs (3,5,11,13), actinomycin D (14), and flavopiridol (7). To understand their mechanisms of action, it i...
In the present study, nested polymerase chain reaction-single-strand conformation polymorphism (nested PCR-SSCP) was used for detection of point mutations in flower color genes in Salvia splendens. Totally 916 bp of Ss5MaT1 cDNA segments were obtained in eight varieties of Salvia splendens flowers by RT-PCR. Four sets of specific primers were synthesized according to the obtained cDNA cloning f...
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