نتایج جستجو برای: denaturing gradient gel electrophoresis
تعداد نتایج: 242037 فیلتر نتایج به سال:
A combination of approaches was used to examine the bacterial communities associated with 2 deep-water Scleritoderma spp. sponges. Denaturing gradient gel electrophoresis analysis of partial 16S rRNA genes extracted from sponge tissue was used to determine the structure of the sponge-associated bacterial community. Cultivation studies using a variety of marine-based media and medium additions w...
A combined lipid biomarker-16S rRNA gene denaturing gradient gel electrophoresis analysis was used to monitor changes in the physiological status, biomass, and microbial composition of a microbial mat. In the morning hours, an increase in the biomass of layers containing a high density of phototrophs and a decrease in the growth rate in the deep layers were observed. The combined approach also ...
Denaturing gradient gel electrophoresis (DGGE) in combination with PCR and 'GC-clamping' has proven highly efficient as a method for detection of DNA sequence differences. Due to strand dissociation phenomena, however, its use has been limited to the analysis of sequences with a relatively low content of GC pairs. This paper describes how treatment of template DNA with sodium bisulphite drastic...
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