A full-length cDNA (LC3'H) was obtained from a cDNA library of Lycoris radiata by DOP-PCR (degenerate oligonucleotide primer PCR), 3'race and 5'race methods. Compared with the other reported enzymes from different plants, the deduced amino acid sequence of LC3'H exhibits significant homologies to 3'-hydroxylases that are involved in the caffeic acid biosynthesis. These findings suggest that the...

Polymerase chain reaction (PCR) is widely applied in clinical and environmental microbiology. Primer design is key to the development of successful assays and is often performed manually by using multiple nucleic acid alignments. Few public software tools exist that allow comprehensive design of degenerate primers for large groups of related targets based on complex multiple sequence alignments...

To develop a physical map, one often uses a large-insert library to establish a contiguous set of overlapping genomic clones (‘contig’). One of the most efficient ways to prepare contigs in limited chromosomal regions is chromosome-walking. For this approach, it is important to retrieve DNA sequences from the termini of the insert fragment to screen for overlapping clones. Many methods for the ...

We have developed a novel coincidence cloning strategy, termed Coincidence Painting, which enables the rapid generation of large numbers of region specific sequences. Coincidence Painting utilises Degenerate Oligonucleotide Primed PCR (DOP-PCR) amplification of flow sorted derivative translocation chromosomes. The PCR products are hybridised in situ onto specific flow sorted chromosomes for coi...

In this paper, the system of Fredholm integral equations of the second kind is investigated by using a modified degenerate kernel  method (MDKM). To construct a MDKM the source function is approximated by the same way of producing degenerate kernel. The interpolation is used to make the needed approximations. Lagrange polynomials are adopted for the interpolation. The equivalency of  proposed m...

‎by considering a degenerate $p(x)-$laplacian equation‎, ‎a generalized compact embedding in weighted variable‎ ‎exponent sobolev space is presented‎. ‎multiplicity of positive solutions are discussed by applying fibering map approach for the‎ ‎corresponding nehari manifold‎.

1-type and biharmonic curves by using laplace operator in lorentzian 3-space arestudied and some theorems and characterizations are given for these curves.