Protein hydrolysate (PH) was prepared from the defatted cottonseed cake by using immobilized or native trypsin on polyamide (nylon 6,6) matrix using glutaraldehyde coupling under the controlled conditions of pH and temperature. Immobilization of trypsin on nylon beads retained 30% of the free enzyme activity and was stable against leaching for more than 30 days. Foaming and emulsification prope...

BACKGROUND Currently there is considerable interest in the potential health benefits of oil seeds, such as soy and flaxseed, especially in relation to cardiovascular disease and cancer. OBJECTIVE We therefore evaluated health aspects of partially defatted flaxseed in relation to serum lipids, indicators of oxidative stress, and ex vivo sex hormone activities. DESIGN Twenty-nine hyperlipidem...

This study optimised the conditions for ultrasound-assisted extraction (UAE) of defatted wheat germ protein (WGP) and evaluated its effect on functional properties. Single-factor orthogonal experiment designs were combined to optimise UAE condition. The WGP reached highest level, at 88.66%, with a solid : liquid ratio 1 : 25 g·mL–1, pH value 9.0, ultrasonic time 10 min, power 400 W. U...

The aim of this work was to compare the antioxidant activity of the extract of flaxseed and its alkaline hydrolysate in two model systems: lipid autoxidation of triacylglycerols of sunflower oil (TGSO)-in a homogeneous lipid media and during β-carotene-linoleate emulsion system. In addition, pure lignans were tested. The material was defatted with hexane and then phenolic compounds were extract...

The objective of this study was to evaluate the fermentation dynamics of 2 commonly fed corn (co)products in their intact and defatted forms, using the in vitro gas production (IVGP) technique, and to investigate the shifts of the predominant rumen bacterial populations using the 16S rDNA bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP) technique. The bTEFAP technique was used to dete...

Malpighia glabra. L (Acerola) fruits have wide global medicinal uses due to rich antioxidant contents. Little studies were carried on other parts of plant. leaves (1.5 kg) was subjected soxhlet exhaustive extraction with methanol and concentrated under vacuum. The dried extract defatted petroleum-ether then fractionated ethyl acetate n-butanol 3 successive times respectively. fraction (9 gm) se...

Aerial parts of Salvia Mirzayanii was extracted with methanol. Methanol extract was suspended in water and defatted with petroleum ether. The defatted part was then partitioned between ethyl acetate and water. The ethyl acetate partition was chromatographed on a silica gel column to afford several fractions. Lymphocyte proliferation inhibitory assay of the resulted fractions was compared in-vit...

Documentation is offered for the identification of 3-O-methyl-mannose as one of several neutral sugars found in defatted arthrospore and mycelial cell walls of Coccidioides immitis.

The authors' previous studies showed that the glycosaminoglycans present in rabbit bone marrow were composed of chondroitin 6-sulfate (79%) and hyaluronic acid (16%). Immunohistochemically the chondroitin 6-sulfate was demonstrated to be in bone marrow matrix constructing hematopoietic microenvironment. In this study the authors isolated and characterized these glycosaminoglycans in their macro...