OBJECTIVE The aim of this study was to investigate whether Quantitative Light-induced Fluorescence-Digital (QLF-D) could monitor the degree of maturation of dental microcosm biofilms by observing the red fluorescence emitted from the biofilms. METHODS Dental microcosm the biofilms were grown on bovine enamel discs. They were initiated from human saliva, and then grown in 0.5% sucrose growth m...

AIMS To investigate (1) whether adequate immunohistochemical staining can be achieved on sections cut from plastic embedded bone marrow trephine biopsy specimens after microwave heating in citrate buffer; and (2) whether this immunohistochemical staining is comparable with that achieved on routine sections cut from paraffin wax embedded trephine biopsy specimens after decalcification procedures...

The structural changes induced by accelerated carbonation in alkali-activated slag/ metakaolin (MK) cements were determined. The specimens were carbonated for 540 h in an environmental chamber with a CO2 concentration of 1.0 ± 0.2%, a temperature of 20 ± 2°C, and relative humidity of 65 ± 5%. Accelerated carbonation led to decalcification of the main binding phase of these cements, which is an ...

Matrix-assisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS) is an advanced method used globally to analyze the distribution of biomolecules on tissue cryosections without any probes. In bones, however, hydroxyapatite crystals make it difficult to determine the distribution of biomolecules using MALDI-IMS. Additionally, there is limited information regarding the use of this...

In August, 1948, at the age of 1 year, this baby girl was admitted to the surgical wards of the Royal Hospital for Sick Children, Glasgow, with a red, hot and painful swelling of the left leg of 10 days' duration. The temperature was 1010 F. and the white blood count 8,000. A diagnosis of haematogenous osteitis was made, the limb was immobilized, and, after removing venous blood for culture, pe...

AIM There are various techniques to study root canal morphology and diaphonization is one of them. There are various methods of decalcification and diaphonization, cited in literature and the main aim of this paper was to give a brief account of the various techniques and share our experience of the technique at a teaching institution in Karachi, Pakistan. MATERIALS AND METHODS Diaphonization...