A fabricação de diferentes nanomaterias com distintas características físico-químicas estão sendo pesquisadas, principalmente nas áreas biológicas e farmacêuticas. As nanopartículas (NPs) são grupos átomos, íons ou moléculas que possuem dimensões entre 1-100 nm. É difundido na pesquisa o uso quitosana (CH), prata (AgNPs) nanotubos carbono (NTC) em bactérias, contudo, as aplicações celulares des...

introduction: acrylamide (acr) known as a neurotoxic agent in human and animals. previous studies have been shown; fasudil improves neurological deficit and neuronal damage. in this study, the effect of fasudil, potent rho-kinase inhibitor, on acr-induced cytotoxicity was evaluated using pc12 cells as a suitable in vitro model. materials and methods: pc12 cells were exposed to different concent...

objective: evaluation of the effects of different doses of antioxidant taurine on oxidative stress and human sperm parameters following cryopreservation. materials and methods: the semen of 20 fertile men were divided to 5 aliquot, one part considered as a fresh after analysis of standard semen parameters (motility, abnormal morphology, viability) and protamine deficiency, dna damage and measu...

Introduction: Acrylamide (ACR) known as a neurotoxic agent in human and animals. Previous studies have been shown; fasudil improves neurological deficit and neuronal damage. In this study, the effect of fasudil, potent Rho-kinase inhibitor, on ACR-induced cytotoxicity was evaluated using PC12 cells as a suitable in vitro model. Materials and Methods: PC12 cells were exposed to different concent...

Background: Cytoplasm and mitochondria are considered as the major origins of sperm ROS production. Sperm is prone to DNA damage by exposure to ROS or due impaired chromatin remodeling or low DNA protamination. Therefore, the aim of this study was to see if there is any association between impaired chromatin packaging and origin of ROS production. Materials and Methods: Cytosolic ROS, mitochond...

Background. Long-term extensive use of glucocorticoids will lead to hormonal necrosis the femoral head, and osteoblasts play an important role in prevention osteonecrosis. However, there is no complete cure for head. Mesenchymal stem cell- (MSCs-) derived exosomes are widely used repair various tissue lesions. Therefore, aim this study was investigate mechanism dexamethasone- (DEX-) induced ost...

A progressive way in photodynamic therapy is to design photosensitive agents that may operate at lower optical power. Hybrid organic–inorganic silver-, iron-, and silver&iron-contained nanoparticles (NPs) were synthesized using H. perforatum extract explored as photosensitizers for anticancer antimicrobial therapies ultra-low power light (2.30 mW/cm2). silver- NPs revealed the most promising re...

Water-soluble and insoluble fractions of airborne particulate matter (PM) exhibit different toxicological potentials peculiar mechanisms action in biological systems. However, most the research on oxidative potential (OP) PM is focused exclusively its water-soluble fraction, since experimental criticisms were encountered for detaching whole (soluble species) from field filters. to estimate actu...

BACKGROUND Hyperlipidemia-induced apoptosis mediated by fatty acid translocase CD36 is associated with increased uptake of ox-LDL or fatty acid in macrophages, hepatocytes and proximal tubular epithelial cells, leading to atherosclerosis, liver damage and fibrosis in obese patients, and diabetic nephropathy (DN), respectively. However, the specific role of CD36 in podocyte apoptosis in DN with ...

Proinflammatory high-density lipoprotein (p-HDL) is a biomarker of cardiovascular disease. Sickle cell disease (SCD) is characterized by chronic states of oxidative stress that many consider to play a role in forming p-HDL. To measure p-HDL, apolipoprotein (apo) B containing lipoproteins are precipitated. Supernatant HDL is incubated with an oxidant/LDL or an oxidant alone and rates of HDL oxid...