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In order to analyze male sterility caused by deletion of SRY and DAZ, we examined the accuracy and cost-effectiveness of a modified primed in situ labeling (PRINS) technique for detection of single-copy genes. Peripheral blood samples were collected from 50 healthy men; medium-term cultured lymphocytes from these samples were suspended in fixative solution and then spread on clean slides. ...

Abstract Background Boule, DAZLA, and DAZ are members of the Deleted in Azoospermia family genes, which play significant roles gametogenesis important fertility factors humans. In a few studies, deletion Y chromosomal cluster single nucleotide polymorphisms DAZLA gene were reported to affect male fertility, although this is paradoxical because they found both control fertile men. As result, goa...

Oxidation of the thiol functional group in cysteine (Cys-SH) to sulfenic (Cys-SOH), sulfinic (Cys-SO2H) and sulfonic acids (Cys-SO3H) is emerging as an important post-translational modification that can activate or deactivate the function of many proteins. Changes in thiol oxidation state have been implicated in a wide variety of cellular processes and correlate with disease states but are diff...

The effects of constrictor prostanoid (CP) pathway inhibitors on vascular reactivity to vasopressin (VP) and phenylephrine (PE) were examined in thoracic aortas of male, female, and ovariectomized (OVX) female Sprague-Dawley rats. Maximal contractile response of control (Cont) aortas to VP was markedly higher in females (3,885 +/- 332 mg/mg ring wt) than in males (810 +/- 148 mg). Indomethacin ...

The superalloy FGH98 was successfully diffusion bonded (DB) with medium-entropy alloy (MEA) Al3Ti3(CrCoNi)94 using pure Ni as the interlayer at a temperature range of 1050–1170 °C for 1 h under 5 MPa. microstructure and mechanical properties joints were investigated. bonding seam composed an zone (IZ) two diffusion-affected zones (DAZ). IZ DAZ beside consisted cubic Ni3(TiAl)-type ?? phases due...

The Y-chromosomal DAZ (deleted in azoospermia) gene and the autosomal Dazl (deleted in azoospermia-like) gene are two crucial factors for the achievement and maintenance of spermatogenesis. Whereas Y-chromosomal DAZ is present in certain primates, it is lacking in rodents and other species. We have investigated the expression of Dazl protein during spermatogenesis in the adult rat testis using ...

The azoospermia factor (AZF) regions consist of three genetic domains in the long arm of the human Y chromosome referred to as AZFa, AZFb and AZFc. These are of importance for male fertility since they are home to genes required for spermatogenesis. In this paper a comprehensive analysis of AZF structure and gene content will be undertaken. Particular care will be given to the molecular mechani...

Our mid-infrared survey of 124 white dwarfs with the Spitzer Space Telescope and the IRAC imager has revealed an infrared excess associatedwith thewhite dwarf WD2115 560 naturally explained by circumstellar dust. This object is the fourth white dwarf observed to have circumstellar dust. All four are DAZ white dwarfs, i.e., they have both photospheric Balmer lines and photospheric metal lines.We...