Mouse mammary tumor virus (MMTV) derived from the culture medium of GR cells contained seven proteins, identified as gp55, gp33, p25, pp20, p16, p12, and p10. The major viral phosphoprotein was the 20,000-molecular-weight protein, pp20. Immunoprecipitation of cytoplasmic extracts from pulse-labeled GR cells identified three MMTV gag-specific proteins, termed Pr78(gag), Pr110(gag), and Pr180(gag...

The core of human immunodeficiency virus type 1 is derived from two precursor polyproteins, Pr55gag and Pr160gag-pol. The Gag precursor can assemble into immature virus-like particles when expressed by itself, while the Gag-Pol precursor lacks particle-forming ability. We have shown previously that the Gag precursor is able to "rescue" the Gag-Pol precursor into virus-like particles when the tw...

in endodontics, severe involuntary gagging can have a severe impact on treatment procedure. there are many ways to ease the gag reflex, one of which is hypnosis. a 34-year-old male was referred for root canal treatment of a molar tooth. he had not received any dental treatments for the past nine years due to fear of severe gag reflex. three hypnotic sessions based upon eye fixation, progressive...

Listeria monocytogenes (Lm) is an attractive vector to elicit T cell immunity because it infects antigen-presenting cells and because infection originates at the mucosa. Lm expressing HIV gag elicits sustained high levels of gag-specific CTL in mice. Since Lm causes disease in immunocompromised hosts, a highly attenuated strain of Lm that requires D-Ala for viability was produced. Attenuated ba...

Design of two RTQPCRs for XMRV-like murine leukemia viruses. © program The variation in MLV gag genes was studied by blasting the nr portion of GenBank with gag of XMRV VP62. BLASTN retrieved 109 MLV gag sequences. The result was treated with the variation analysis program ConSort (Blomberg et al, unpublished) (fig S1 A and B). The selected portion of gag corresponded approximately to the major...

Interactions of human immunodeficiency virus type 1 (HIV-1) Vif protein with various forms of Gag and Gag-Pol precursors expressed in insect cells were investigated in vivo and in vitro by co-encapsidation, co-precipitation and viral protease (PR)-mediated Gag processing assays. Addressing of Gag to the plasma membrane, its budding as extracellular virus-like particles (VLP) and the presence of...