It was suggested by previous workers that the C-26 oxygenation step in the biosynthetic conversion of cholesterol to tigogenin ((25R)-5a,220-spirostan-3-ol) requires the intermediacy of a C-24 double bond. To examine the validity of this hypothesis, (25-3H)-cholesterol was synthesized, and (4-'4 C)-(253 H)-cholesterol (H:14C ratio, 7.0) was administered to two Digitalis samen plants. A method o...

Supplementation of dairy rations with steroidal sapogenins was evaluated in an in vitro, an in situ, and a production trial. Sixteen in vitro semicontinuous rumen cultures were supplied a 55:45 concentrate to roughage substrate with Sarsaponin concentrations of 0, 33, 55, and 77 ppm of air-dry feed for 22 d. Supplementation decreased protozoa numbers and increased bacterial numbers and acid det...

Identifying novel phytochemical secondary metabolites following classical pharmacognostic investigations is tedious and often involves repetitive chromatographic efforts. During the past decade, Ultra-High Performance Liquid Chromatography-Quadrupole Time of Flight-Tandem Mass Spectrometry (UHPLC-QToF-MS/MS), in combination with molecular networking, has been successfully demonstrated for rapid...