A novel enzyme, alpha-neoagarooligosaccharide hydrolase (EC 3.2.1.-), which hydrolyzes the alpha-1,3 linkage of neoagarooligosaccharides to yield agaropentaose (O-beta-D-galactopyranosyl(1-->4)-O-3,6-anhydro-alpha-L-galactopyranosyl (1-->3)-D-galactose], agarotriose [O-beta-D-galactopyranosyl(1-->4)-O-3,6-anhydro- alpha-L-galactopyranosyl (1-->3)-D-galactose], agarobiose [O-beta-D-galactopyrano...

This study aimed to prepare the total saponins from Panax japonicus (TSPJ), and investigate their protective effects on learning memory ability of aging mice induced by D-galactose. The TSPJ product with content 81.03% was obtained. Fifty were randomly divided into control, model, low-, middle- high-dose groups. D-galactose-induced model established in Then, groups treated 20, 40 80 mg/kg gavag...

Some of the properties of a 70-fold purified bovine milk lactose synthetase have been determined. The enzyme preparation appears to be specific for uracil nucleoside diphosphate D-galactose derivatives. Adenosine, thymidine, @dine, or guanosine diphosphate derivatives cannot serve as galactosyl donors for n-glucose to form lactose. The enzyme preparation will not transfer the galactosyl residue...

Galactose transport was studied in membrane vesicles, prepared by fusion of plasma membranes from the yeast Kluyveromyces marxianus with proteoliposomes containing beef heart cytochrome c oxidase as a proton-motive force-generating system. Sugar transport studies performed under nonenergized conditions revealed that, even at high protein to phospholipid ratios, not all vesicles contained a D-ga...

The capsular polysaccharide of Diplococctw pneumoniae Type XII contains residues of D-glucose and D-galactose in a molar ratio of 2:l. The methylated polysaccharide yielded upon hydrolysis 2,3,4,6-tetraand 3,4,6-tri-O-methyl-D-glucose and 2,3,4,6-tetra-0-methyl-D-galactose as the only neutral methyl sugars. Periodate oxidation of the polysaccharide resulted in destruction of all neutral sugars ...

Anti-oxidative, anti-glycative and anti-apoptotic effects of oleanolic acid in brain from D-galactose treated mice were examined. Oleanolic acid at 0.05%, 0.1% and 0.2% was supplied to mice for 10 wk. D-Galactose treatment increased reactive oxygen species and protein carbonyl levels (P<0.05), and reduced activity and protein production of glutathione peroxide, superoxide dismutase and catalase...

In order to develop potential anti-fungal agents, seven glycoconjugates composed of α-L-rhamnose, 6-deoxy-α-L-talose, β-D-galactose, α-D-mannose, β-D-xylose-(1→4)-6-deoxy-α-L-talose, β-D-galactose-(1→4)-α-L-rhamnose, β-D-galactose-(1→3)-β-D-xylose-(1→4)-6-deoxy-α-L-talose as the glycone and oleanolic acid as the aglycone were synthesized in an efficient and practical way using glycosyl trichlor...

The L-arabinose-binding protein (ABP) of Escherichia coli consists structurally of two distinct globular domains connected by a hinge of three separate peptide segments. Arabinose is bound and completely sequestered within the deep cleft between the two domains. With reduced affinity, ABP also binds D-galactose (approximately 2-fold reduction) and D-fucose (approximately 40-fold reduction). Exp...