The present study was conducted to induce callus from different in vitro grown seedling explants of shoot, root and leaf segments in order to develop shoot buds and rooting from callus. The sterilized seeds were cultured on Murashige and Skoog medium, and then the explants were cultured from seedling and transferred to an MS medium supplemented with different concentrations of BAP, kinetin, NAA...

[a]pyrene (BaP), a polycyclic aromatic hydrocarbon (PAH) and animal carcinogen, is used by regulators as the basis for quantitative risk estimation of PAH-containing combustion by-products (1). Is BaP an appropriate predictor of cancer risk for products of incomplete combustion? A multidose, 2-year (chronic) feeding protocol developed by the National Institute of Environmental Health Sciences i...

Benzo[a]pyrene (BaP) is a human carcinogen requiring metabolic activation prior to reaction with DNA. Cytochrome P450 (CYP) 1A1 is the most important hepatic and intestinal enzyme in both BaP activation and detoxification. CYP1A2 is also capable of oxidizing BaP, but to a lesser extent. The induction of CYP1A1/2 by BaP and/or β-naphthoflavone in liver and small intestine of rats was investigate...

Metabolism of benzo(a)pyrene (BaP) in vivo and in vitro was studied using two benthic fish species, English sole (Parophrys vetulus) and starry flounder (Platichthys stellatus), and Sprague-Dawley rats. At 24 h after administration of BaP (7.9 mumol/kg of body weight) to fish either p.o. (Experiment 1) or i.p. (Experiment 2), the specific activity of binding of BaP metabolites to hepatic DNA (p...

Background: Bituminaria bituminosa is a long-day plant. The plant has high pastoral value and palatability. It grazed by cattle, goats sheep. Leaves stems contain four furanocoumarins’ psoralen, angelicin, xanthotoxin bergapten. seed covered the fruit. Germination was not observed in beaked fruits. A cut with scalpel can increase germination rate. According to literature there are enough studie...

The present study was the first report on somatic embryogenesis of Tunisian chickpea (Cicer arietinum L.) particularly supposed to be recalcitrant and difficult manipulate in vitro. An efficient protocol has been developed for inducing indirect derived from immature zygotic embryo axis, young leaflet hypocotylar explants. Callogenesis achieved full-strength Murashige Skoog's (1962) (MS) basal m...

Rice (Oryza sativa L.) is the world’s most important cereal crop. The Doairgura variety (O. a low-yielding, high-quality aromatic rice-producing in Bangladesh. This study was undertaken to develop an vitro regeneration protocol through callus induction. MS media supplemented with different concentrations of 2, 4- D (0.5, 1.0, 2.0 and 3.0 mg /l) were used for induction from mature dehusked rice ...

Digitalis davisiana, commonly called Alanya foxglove, from Turkey, is an important medicinal herb as the main source of cardiac glycosides, cardenolides, anthraquinones, etc. It is also known in the Indian Medicine for treatment of wounds and burns. It has ornamental value as well. Overexploitation of D. davisiana has led this species to be declared protected, and thereby encouraged various met...

Benzo[a]pyrene (BaP) is a by-product of incomplete combustion of fossil fuels and plant/wood products, including tobacco. A physiologically based pharmacokinetic (PBPK) model for BaP for the rat was extended to simulate inhalation exposures to BaP in rats and humans including particle deposition and dissolution of absorbed BaP and renal elimination of 3-hydroxy benzo[a]pyrene (3-OH BaP) in huma...

Abstract Pranajiwa (Euchresta horsfieldii (Lesch) Benn.) is an endangered medicinal plant known for its function as aphrodisiac, antidote, expectorant, and tonic. It also can be used to cure TBC. Research has found that pranajiwa difficult propagated, but the tissue culture propagation method never been applied this species. This research aimed figure out procedure induce callogenesis of with a...