gene in human glioma cell lines. O-6-methylguanine DNA methyltransferase factors from and suppressed expression of the associated with exclusion of transcription Methylation-related chromatin structure is

Methylation of ribose moieties appears to be an essential post-transcriptional event in ribosomal RNA maturation. Although the sites of ribose methylation have been identified, the components involved in the 2'-O-methylation of precursor ribosomal RNA in mammalian cells have not yet been elucidated. To investigate the involvement of a recently isolated nucleolar 2'-O-methyltransferase in this p...

The plus-strand RNA genome of flavivirus contains a 5' terminal cap 1 structure (m7GpppAmG). The flaviviruses encode one methyltransferase, located at the N-terminal portion of the NS5 protein, to catalyze both guanine N-7 and ribose 2'-OH methylations during viral cap formation. Representative flavivirus methyltransferases from dengue, yellow fever, and West Nile virus (WNV) sequentially gener...

Although an enormous and still growing number of biologically diverse methyltransferases have been reported and identified, a comprehensive understanding of the enzymatic methyl transfer mechanism is still lacking. Glycine N-methyltransferase (GNMT), a member of the family that acts on small metabolites as the substrate, catalyzes methyl transfer from S-adenosyl-l-methionine (AdoMet) to glycine...

Ocimum selloi Benth, a native plant of Brazil, has medicinal uses as anti-diarrheic, antispasmodic and anti-inflammatory product. The yield of essential oils of the inflorescences, containing flowers and seeds, was 0.6%, and the yield of leaves, collected in two different seasons, was 0.25% (June 2000) and 0.20% (January 2001), respectively. The essential oils of the inflorescences and leaves p...

IFIT (interferon-induced with tetratricopeptide repeats) proteins are critical mediators of mammalian innate antiviral immunity. Mouse IFIT1 selectively inhibits viruses that lack 2'O-methylation of their mRNA 5' caps. Surprisingly, human IFIT1 does not share this antiviral specificity. Here, we resolve this discrepancy by demonstrating that human and mouse IFIT1 have evolved distinct functions...

Biogenic terpenes play important roles in ecosystem functioning and atmospheric chemistry. Some of these compounds are semi-volatile and highly reactive, such as sesquiterpenes and oxygenated terpenes, and are thus difficult to quantify using traditional air sampling and analysis methods. We developed an alternative approach to quantify emissions from live branches using a flow through enclosur...

A microsomal fraction from flax hypocotyls (Linum usitatissimum L) showed a methylation ability from S-adenosyl-methionine on to the cell wall polysaccharides. Two kinds of methylation were found: (i) a methyl esterification of uronic acids in the oxalate extracts and (ii) an O-methylation of the hydroxyl groups in the NaOH extracts. The methyltransferase study showed a rapid decrease of the me...