Leishmaniasis is a neglected tropical disease infecting world’s poorest population in over 90 countries throughout Asia, Africa, the Middle East, and Central South Africa. An underestimated 700,000 to one million new cases occur annually. refers spectrum of diseases caused by parasite, Leishmania, transmitted bite infected sandflies. Of three major syndromes (cutaneous, mucosal visceral) leishm...

We report our experience with the diagnosis and treatment o f 60 patients with American cutaneous leishmaniasis. They were infected in Panama (55), Brazil (4) or Colombia (I). Among 35 patients with a 3 week exposure in Panama, the mean maximum incubation period was 33 days (range 4-81 days). Diagnosis was delayed an average o f 93 days after onset o f skin lesions, due to the patient’s delay i...

cutaneous leishmaniasis is one of the most important parasitic diseases, which are endemic in different parts of iran.   leishmania major and l. tropica are the primary causative agents of this disease. the aim of the present study was to detect the multiple forms of l. major in lung. ppromastigotes of l. major at stationary phase were injected to balb/c mice. after 60 days, the different forms...

We report four disseminated cutaneous leishmaniasis(DCL) cases referred to leishmaniasis laboratory at the School of Public Health, Tehran University of Medical Sciences with multiple nodular, ulcerative and crusted lesions extended on the face, trunk, and extremities. None of the patients had any complication and historical involvement in their immunological system conditions that suggest as t...

The present report describes a real-time PCR-based procedure to reliably determine the quantity of Leishmania amastigotes in relation to the amount of host tissue in histological skin sections from canine and equine cases of cutaneous leishmaniasis. The novel diagnostic Leishmania-PCR has a detection limit of <0.02 amastigotes per μg tissue, which corresponds well to the detection limit of immu...

We report on a 37-year-old male HIV-positive patient with generalized cutaneous leishmaniasis undiagnosed for several years. Upon presentation, visceral leishmaniasis was diagnosed in addition to cutaneous manifestation of the disease. Over a period of three years, several different treatment regimens including liposomal amphotericin B, liposomal amphotericin B with miltefosine, liposomal ampho...

OBJECTIVE The objective of this study was to analyze different primers that are commonly used in epidemiological studies for the detection of Leishmania DNA by PCR, and to compare them to the conventional direct parasite search for American cutaneous leishmaniasis (ACL) diagnosis. MATERIAL AND METHODS Five pairs of primers, four of them derived from Leishmania kDNA sequences (MP3H-MP1L; B1-B2...

BACKGROUND Lutzomyia umbratilis, the vector for Leishmania guyanensis in northern South America, has been found naturally infected with L. guyanensis only in areas north of the Negro and Amazon rivers. While populations of this sand fly species are also found in areas south of these rivers, these populations have never been reported to be infected and/or transmitting L. guyanensis. However, no ...

In a recent issue of the Journal of Clinical Microbiology, Ryan et al. (6) reported antileishmanial serologic data based on soluble antigens from promastigotes cultivated in a proteinfree medium. Using the enzyme-linked immunosorbent assay (ELISA), it was possible to detect immunoglobulin M (IgM) and IgG antibody in patients with cutaneous leishmaniasis (143 sera) and visceral leishmaniasis (12...

In Colombia, zosteriform leishmaniasis is a little-known and infrequent clinical variant of cutaneous leishmaniasis. Its clinical features include one or more plaques made up of papules and pseudo-vesicles, which conform to a lineal pattern, as well as satellite lesions that affect one or more dermatomes, without crossing the median line. We present three zosteriform cutaneous leishmaniasis cas...