Project abstract: In this work, a way to cell-culture real-time monitoring system by means of the Oscillation-Based Test (OBT) methodology [1]is proposed and implemented with Texas Instrument (TI) components. The idea is inspired in previous works from the authors [1] in the area of testing analogue integrated circuits and deals with solving some critical points in this kind of biological measu...

Ethnomathematics is the study of mathematics' distinctive characteristics or colors as they exist and develop in society. mathematics it appears utilized cultural community groups such urban rural communities, children a specific age, labor indigenous peoples. For vast majority Indonesians, cultivation way life, particularly residents Nanga Mahap Village. Mathematical words are employed farming...

The sparse connectivity within the striatum in vivo makes the investigation of individual corticostriatal synapses very difficult. Most studies of the corticostriatal input have been done using electrical stimulation under conditions where it is hard to identify the precise origin of the cortical input. We have employed an in vitro dissociated cell culture system that allows the identification ...

There are innumerable varieties of devices available for the supply of fluid media to continuous culture systems. The unit presented here offers the advantage of being an inexpensive system with a high degree of reliability. No moving parts or pumps are required, and functional models can be constructed from routine laboratory ware. The primary problem with gravity flow or "drip" systems has be...

A self-contained mechanical system for circulating nutrient fluid through 12 tissue culture chambers is described in detail. This system utilizes nonperforated cellophane membranes in the chambers which separate the circulating nutrient from the tissue culture environments. The nutrient, therefore, is dialyzed through the cellophane of each chamber; some cell products are retained in the microe...

In a comparative fungal blood culture study, a lysis-centrifugation system (Isolator; Du Pont Co., Wilmington, Del.) detected 89% of all episodes of fungemia; the lysis-centrifugation system detected fungemia exclusively or significantly earlier than did a biphasic brain heart infusion bottle system 83% of the time. The lysis-centriguation system was particularly useful in the early detection o...

Unraveling the molecular mechanisms of lymphangiogenesis is hampered by the lack of appropriate in vitro models of three-dimensional (3D) lymph vessel growth which can be used to exploit the potential of available transgenic mice. We developed a potent reproducible and quantifiable 3D-culture system of lymphatic endothelial cells, the lymphatic ring assay, bridging the gap between 2D-in vitro a...

There is increasing demand for automated and quantitative cell culture technology, driven both by the intense activity in stem cell biology and by the emergence of systems biology. We built a fully automated cell culture screening system based on a microfluidic chip that creates arbitrary culture media formulations in 96 independent culture chambers and maintains cell viability for weeks. Indiv...

objective adult stem cells which are derived from different tissues, with their unique abilities to self-renew and differentiate into various phenotypes have the potential for cell therapy and tissue engineering. human adipose tissue is an appropriate source of mesenchymal stem cells with wide differentiation potential for tissue engineering research. in this study isolated stem cells from huma...