Objective Chlamydia trachomatis (CT) is the most common sexually transmitted infection (STI) and can lead to pelvic inflammatory disease, infertility, and chronic pelvic pain in women and increased risk of vertical transmission, conjunctivitis, and pnuemonitis in infants. It may also be a co-factor along with HPV in cervical cancer progression. The current study aimed to determine the prevalenc...

A cationic platinum(II) complex (1), [Pt(4-NOIP)(en)]·Cl2 (4-NOIP = 2-(4-nitro)-imidazo-[5,6-f][1,10]-phenanthroline, en = 1,2-ethylenediamine), has been synthesized and characterized by IR, UV-vis, ESI-MS and element analysis. The interaction of complex (1) with ct-DNA and human telomeric G-quadruplex DNA [Htel-21] has been investigated by circular dichroism absorption and UV–vis absorption ti...

Today, a consensus that DNA working as a molecular wire shows promise in nanoscale electronics is reached. Considering that the "ON-OFF" switch is the basis of a logic circuit, the switch of DNA-mediated charge transport (DNA CT) should be conquered. Here, on the basis of chemical or electrochemical deposition and stripping of DNA-templated copper nanoclusters (CuNCs), we develop an "ON-OFF" na...

Sensitive and specific tests for detecting exogenous DNA molecules are useful for infectious disease diagnosis, gene therapy clinical trial safety, and gene doping surveillance. Taqman real-time PCR using specific sequence probes provides an effective approach to accurately and quantitatively detect exogenous DNA. However, one of the major challenges in these analyses is to eliminate false posi...

introduction: vaccinology provides promising approaches for the control of various infectious diseases. among different strategies, dna vaccines offer attractive research opportunities for development of vaccines for induction of antigen-specific immunity owing to their stability, simplicity of delivery, safety and cost effectiveness. however, there is a need to increase their potency by the us...

PURPOSE Computed tomography (CT) scans are an important source of ionizing irradiation (IR) in medicine that can induce a variety of DNA damage in human tissues. With technological improvements CT scans at reduced absorbed doses became feasible presumably lowering genotoxic side effects. MATERIALS AND METHODS For measuring DNA damage we performed γH2AX foci microscopy in peripheral blood mono...

A charge transport (CT) mechanism has been proposed in several articles to explain the localization of base excision repair (BER) enzymes to lesions on DNA. The CT mechanism relies on redox reactions of iron-sulfur cofactors that modify the enzyme's binding affinity. These redox reactions are mediated by the DNA strand and involve the exchange of electrons between BER enzymes along DNA. We prop...

In this study, aggregation behavior of a water soluble cationic metalloporphyrin, meso-tetrakis (2,3,5,6-tetrafluoro-N,N´,N´´-trimethyl ammonium phenyl) porphyrin nickel(II) [Ni(II)(TF4TMAPP)]4+ is investigated in 5 mM aqueous phosphate buffer of pH 7.0 at 25.0 °C and various ionic strengths using optical absorption and resonance light scattering spectroscopic methods. The...

UNLABELLED This report describes gene transfer in vitro as well as in vivo using cetylated low-molecular mass (600 Da) polyethylenimine (28% of amine groups substituted with cetyl moieties), termed CT-PEI. This compound is hydrophobic and has to be incorporated into liposomes in order to be suitable for gene transfer studies. Serum-induced plasmid DNA degradation assay demonstrated that CT-PEI-...

In depth interaction studies between calf thymus deoxyribonucleic acid (CT-DNA) and a series of four structurally relative palladium(II) complexes [Pd(en)(HB)](NO3)2 (a-d), where en is ethylenediamine and heterocyclic base (HB) is 2,2'-bipyridine (bpy, a); 1,10-phenanthroline (phen, b); dipyridoquinoxaline (dpq, c) and dipyridophenazine (dppz, d) (Figure 1), were performed. These studies have b...