With the aim of the secretion of human granulocyte macrophage-colony stimulating factor (hGM-CSF) in Escherichia coli, hGM-CSF cDNA was fused in-frame next to the signal sequence of ST toxin (ST-I) of exteroxigenic E. coli, containing 53 or 19 amino acids of signal peptide. The fused STsig::hGM-CSF coding fragments were inserted into a T7-based expression plasmid. The recombinant plasmids were ...

macrophage colony stimulating factor (m-csf) has previously been shown to affect the differentiation of cells of the mono-nuclear phagocytic line. more recent studies indicate that m-csf may have a role in pregnancy. in the present study, the expression of m-csf in the human placenta was demonstrated. placental mrna was isolated and used as template for synthesis of complementary dna (cdna). th...

The functions of the glymphatic system include clearance metabolic waste and modulation water transport in brain, it forms a brain-wide fluid network along with cerebrospinal (CSF) interstitial (ISF). pathway consists periarterial influx CSF, astrocyte-mediated interchange between ISF CSF supported by aquaporin-4 (AQP4) on endfeet astrocyte around periarterioles, perivenous efflux CSF. Finally,...

Introduction: Following our previous studies on the neuroprotective effects of progesterone and cytokines such as IL-6 (IL-6) is involved in the inflammatory response, to examine whether changes in IL-6 and Nerve grows factor(NGF) concentrations in CSF can responsible for the neuroprotective effects of progesterone after traumatic brain injury(TBI). Materials and Methods: Female ovariectomized ...

Ovarian torsion is an important reproductive gynecological emergency in daily life and physiology. Granulocyte colony stimulating factor (G-CSF) a glycoprotein capable of hematopoietic development successfully used the treatment congenital granulocytopenia. In our study, we aimed to investigate protective effects G-CSF on ovarian ischemia reperfusion (IR) immunohistochemically. Four groups were...

Studi epidemiologis tentang kejadian CSF penting untuk lebih memahami faktor risiko dan menentukan riwayat alami kondisi tersebut. Oleh karena itu , systematic review ini dibuat membahas insiden terjadinya CSF, serta mengetahui berbagai prediktor yang terkait dengan CSF. Tinjauan sistematis dianalisis menggunakan metode PRISMA-P 2015. Berdasarkan hasil pencarian, terdapat 10 artikel relevan mem...

bacterial expression systems provide economic and logistic advantages in manufacturing proteins for human therapeutic purposes. however, most such proteins accumulate in insoluble biologicaiiy inactive form when overexpressed in bacterial cells. this is the case while attempting to produce recombinant human granulocyte-macrophage colony stimulating factor (hgm-csf) in e. coli. in this report, d...