BACKGROUND If it were possible to deduce the mechanism of injury in cryopreserved embryos by their appearance, it would help to optimize cryopreservation protocols. METHODS Mouse blastocysts were treated so that they were damaged by the six types of cryoinjuries listed below, and their appearance was observed at recovery in sucrose solution and a modified phosphate-buffered saline (PB1), and ...

From a clinical point of view, it is very important to control the ice ball growth in view of sparing healthy tissues or vital structures such as the urethral lining, as well as to increase cryoinjury as much as possible to cancerous tissues. This paper is concerned with the thermal analysis of the freezing of a prostate using a multi-cryoprobe system including a urethral warmer. We address the...

PURPOSE Although cryotherapy has long been used to eradicate corneal lesions, there have been no reports of adverse effects of cryotherapy on human corneas. We performed this study to evaluate and characterize ultrastructural damage to the human cornea following the transcorneal freezing-and-thawing procedure. METHODS Seven human donor corneas were randomly divided into three groups. 1, 2, an...

BACKGROUND Autologous bone marrow cells (BMCs) transplanted into ventricular scar tissue may differentiate into cardiomyocytes and restore myocardial function. This study evaluated cardiomyogenic differentiation of BMCs, their survival in myocardial scar tissue, and the effect of the implanted cells on heart function. METHODS AND RESULTS IN VITRO STUDIES BMCs from adult rats were cultured ...

The paucity of mammalian adult cardiac myocytes (CM) proliferation following myocardial infarction (MI) and the remodeling of the necrotic tissue that ensues, result in non-regenerative repair. In contrast, zebrafish (ZF) can regenerate after an apical resection or cryoinjury of the heart. There is considerable interest in models where regeneration proceeds in the presence of necrotic tissue. W...

The larva of the drosophilid fly, Chymomyza costata, is probably the most complex metazoan organism that can survive submergence in liquid nitrogen (-196 °C) in a fully hydrated state. We examined the associations between the physiological and biochemical parameters of differently acclimated larvae and their freeze tolerance. Entering diapause is an essential and sufficient prerequisite for att...

OBJECTIVE To develop electron paramagnetic resonance (EPR) oximetry as a tool to characterize the oxygen environment in human ovarian xenografts in the early postgrafting period. DESIGN Prospective experimental study. SETTING Gynecology research unit in a university hospital. PATIENT(S) Biopsies were obtained from 6 women aged 22-35 years. INTERVENTION(S) Frozen-thawed human ovarian tis...