نتایج جستجو برای: cryo

تعداد نتایج: 7054  

Journal: :Structure 1999
J Harms A Tocilj I Levin I Agmon H Stark I Kölln M van Heel M Cuff F Schlünzen A Bashan F Franceschi A Yonath

BACKGROUND Ribosomes are the universal cellular organelles that accomplish the translation of the genetic code into proteins. Electron cryo-microscopy (cryo-EM) has yielded fairly detailed three-dimensional reconstructions of ribosomes. These were used to assist in the determination of higher resolution structures by X-ray crystallography. RESULTS Molecular replacement studies using cryo-EM r...

Journal: :Ophthalmology 2016
Graham E Quinn Charles Barr Don Bremer Rae Fellows Alice Gong Robert Hoffman Michael X Repka Jennifer Shepard R Michael Siatkowski Kelly Wade Gui-Shuang Ying

PURPOSE To compare infant and retinopathy of prematurity (ROP) characteristics from 3 clinical studies conducted over a 27-year period in the United States. DESIGN Secondary analysis of results of 3 clinical studies. PARTICIPANTS Infants with birth weight (BW) <1251 g. METHODS Analysis of data from the Cryotherapy for Retinopathy of Prematurity (CRYO-ROP) and Early Treatment for Retinopat...

Journal: :Plant physiology 2000
H Cochard C Bodet T Améglio P Cruiziat

The current controversy about the "cohesion-tension" of water ascent in plants arises from the recent cryo-scanning electron microscopy (cryo-SEM) observations of xylem vessels content by Canny and coworkers (1995). On the basis of these observations it has been claimed that vessels were emptying and refilling during active transpiration in direct contradiction to the previous theory. In this s...

2014
Purbasha Sarkar Elena Bosneaga Edgar G. Yap Jyotirmoy Das Wen-Ting Tsai Angelo Cabal Erica Neuhaus Dolonchampa Maji Shailabh Kumar Michael Joo Sergey Yakovlev Roseann Csencsits Zeyun Yu Chandrajit Bajaj Kenneth H. Downing Manfred Auer

Cost-effective production of lignocellulosic biofuel requires efficient breakdown of cell walls present in plant biomass to retrieve the wall polysaccharides for fermentation. In-depth knowledge of plant cell wall composition is therefore essential for improving the fuel production process. The precise spatial three-dimensional (3D) organization of cellulose, hemicellulose, pectin and lignin wi...

2013
Xiao-chen Bai Israel S Fernandez Greg McMullan Sjors HW Scheres

Although electron cryo-microscopy (cryo-EM) single-particle analysis has become an important tool for structural biology of large and flexible macro-molecular assemblies, the technique has not yet reached its full potential. Besides fundamental limits imposed by radiation damage, poor detectors and beam-induced sample movement have been shown to degrade attainable resolutions. A new generation ...

Journal: :Journal of structural biology 2012
Wen Jiang Fei Guo Zheng Liu

Accurate determination of micrograph focuses is essential for averaging multiple images to reach high-resolution 3-D reconstructions in electron cryo-microscopy (cryo-EM). Current methods use iterative fitting of focus-dependent simulated power spectra to the power spectra of experimental images, with the fitting performed independently for different images. Here we have developed a novel graph...

2001
H. Quack M. Kauschke C. Haberstroh H. Lierl B. Petersen S. Wolff

The Tera-eV Energy Superconducting Linear Accelerator (TESLA) is a 32 km long superconducting linear electron/positron collider of 500 GeV centre of mass energy (upgradeable to 800 GeV), presently in the planning phase at DESY [1,2]. About 21000 superconducting RF 9-cell cavities have to be cooled with superfluid helium at 2 K. The cavities are assembled in groups of 12 into about 16 m long cry...

Journal: :Biochemical Society transactions 2013
Karen M Davies Bertram Daum

To truly understand bioenergetic processes such as ATP synthesis, membrane-bound substrate transport or flagellar rotation, systems need to be analysed in a cellular context. Cryo-ET (cryo-electron tomography) is an essential part of this process, as it is currently the only technique which can directly determine the spatial organization of proteins at the level of both the cell and the individ...

Journal: :Biology of the cell 2003
Cedric Messaoudi Thomas Boudier Jean-Pierre Lechaire Jean-Louis Rigaud Hervé Delacroix Françoise Gaill Sergio Marco

Recent advances in electron microscopy and image analysis techniques have resulted in the development of tomography, which makes possible the study of structures neither accessible to X-ray crystallography nor nuclear magnetic resonance. However, the use of tomography to study biological structures, ranging from 100 to 500 nm, requires developments in sample preparation and image analysis. Inde...

Journal: :Nature Communications 2021

Abstract Cryo-electron microscopy (cryo-EM) maps usually show heterogeneous distributions of B -factors and electron density occupancies are typically -factor sharpened to improve their contrast interpretability at high-resolutions. However, ‘over-sharpening’ due the application a single global can distort processed causing connected densities appear broken disconnected. This issue limits cryo-...

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