Contact sites in interaction between light-activated rhodopsin and transducin (T) have been investigated by using a chemically preactivated crosslinking reagent, N-succinimidyl 3-(2-pyridyldithio)propionate. The 3 propionyl-N-succinimidyl group in the reagent was attached by a disulfide exchange reaction to rhodopsin mutants containing single reactive cysteine groups in the cytoplasmic loops. C...

This study focuses on determining the effect of varying the composition and crosslinking of collagen-based films on their physical properties and interaction with myoblasts. Films composed of collagen or gelatin and crosslinked with a carbodiimide were assessed for their surface roughness and stiffness. These samples are significant because they allow variation of physical properties as well as...

We have investigated changes in membrane protein organization of ATPdepleted red cells by employing intermolecular crosslinking of the neighboring membrane proteins with glutaraldehyde or catalytic oxidation. After oxidative crosslinking, the complexes of crosslinked proteins were separated by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS PAGE) and treated with a reducing agent,...

We have investigated changes in membrane protein organization of ATPdepleted red cells by employing intermolecular crosslinking of the neighboring membrane proteins with glutaraldehyde or catalytic oxidation. After oxidative crosslinking, the complexes of crosslinked proteins were separated by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS PAGE) and treated with a reducing agent,...

Gliadin was reacted with citric acid under weak acidic and weak alkaline conditions in both wet and dry states and the reaction mechanism was studied. The low morphological stability in an aqueous environment and inferior mechanical properties have restricted the applications of plant proteins, although these materials possess a unique structure, biocompatibility and biodegradability. Carboxyli...

We report a facile new route for the synthesis of self-crosslinked anion exchange membranes (AEM) without the need for any crosslinkers or catalysts. The soluble copolymers bearing flexible side chains, with alkene pendant groups, were synthesized via the Menshutkin reaction. The crosslinked derivatives were then prepared by the thermal crosslinking of the unsaturated side chains during the mem...

For many paramyxoviruses , including Newcastle disease virus (NDV), syncytia formation requires the expression of both surface glycoproteins (HN and F) in the same cell , and evidence suggests that fusion involves a specific interaction between the HN and F proteins (23 , 73). Because a potential interaction in paramyxovirus infected cells has never been clearly demonstrated , such an interacti...

The application of the microencapsulation technique in medicine and pharmacy has been extensively studied in recent years. The microencapsulation of small particles in polymeric, waxy or other kind of protective shells has become a well-established technology for coating and isolating various substances. The present paper presents the results obtained in a study concerning the influence of cros...

The study was carried out to develop procedure for determining concentration of formaldehyde to be used for crosslinking of gelatin in the presence of drugs having amino groups. Gentamicin sulfate was used as a drug candidate due to its high content of amino acids. Gelatin crosslinking is accelerated by aldehyde-containing compounds and inhibited by amino group-containing compounds. The major m...