We investigated the potential superantigenic properties of Clostridium perfringens enterotoxin (CPE) on human peripheral blood mononuclear cells (PBMC). In contrast to the findings of a previous report (P. Bowness, P. A. H. Moss, H. Tranter, J. I. Bell, and A. J. McMichael, J. Exp. Med. 176:893-896, 1992), two different, biologically active preparations of CPE had no mitogenic effects on PBMC. ...

1. Eighth IEEE International Conference Grid 2007. Austin, TX, 19–21 September 2007. Available at: http://www.grid2007.org/. 2. Carroll TE, Grosu D. Formation of virtual organizations in grids: A game-theoretic approach. DOI: 10.1002/cpe.1369. 3. Macias M, Rana OF, Smith G, Guitart J, Torres J. Maximising revenue in grid markets using an economically enhanced resource manager. DOI: 10.1002/cpe....

Claudin, a tight junction integral membrane protein and a family of proteins, forms the actual sealing element of the tight junction. There are more than 20 members of the claudin family with different tissue-specific expression and barrier functions. Thus, a family of claudin may be a target for modifying the absorption of drugs. Here, we examined whether modulation of claudin could be used to...

A novel electronic cell sensor array technology, the real-time cell analysis (RTCA) system, was developed to monitor cell events. Unlike the conventional methods labeling the target cells with fluorescence, luminescence, or light absorption, the RTCA system allows for label-free detection of cell processes directly without the incorporation of labels. Here, we used this new format to measure th...

Treatment of cultured bovine adrenal chromaffin cells with the catecholamine transport blocker reserpine was shown previously to increase enkephalin levels severalfold. To explore the biochemical mechanism of this effect, we examined the effect of reserpine treatment on the activities of three different peptide precursor processing enzymes: carboxypeptidase E (CPE) and the prohormone convertase...

BACKGROUND The moderately homologous (approx. 60%) proteins Ara h 2 and Ara h 6 are the most potent peanut allergens. This study was designed to define the relative individual contributions of Ara h 2 and Ara h 6 to the overall allergenic activity of a crude peanut extract (CPE). METHODS Ara h 2 and Ara h 6 were removed from CPE by gel filtration chromatography. Ara h 2.01, Ara h 2.02 and Ara...

A new method was developed for simultaneous determination of copper, lead, and cadmium, based on their voltammetric response at a carbon paste electrode modified with hexagonal mesoporous silica (HMS) immobilized quercetin (HMS-Qu/CPE). Compared with quercetin modified carbon paste electrode (Qu/CPE) and quercetin ionic liquid modified carbon paste electrode (Qu-IL/CPE), the HMS-Qu/CPE exhibite...

To learn users’ preference, their feedback information is commonly modeled as scalars and integrated into matrix factorization (MF) based algorithms. Based on MF techniques, the preference degree is computed by the product of user and item vectors, which is also represented by scalars. On the contrary, in this paper, we express users’ feedback as constrained vectors, and call the idea constrain...

Four methods for the assay of human interferon in Vero cells were compared based on the inhibition of viral cytopathic effect (CPE) in tubes, the inhibition of CPE in microplates, the reduction of plaques, and the inhibition of quantitative hemadsorption. For inhibition of CPE, Sindbis virus, vesicular stomatitis virus, poliovirus type 2, and vaccinia virus were used for challenge. In the plaqu...

An active film was prepared by incorporating cortex Phellodendron extract (CPE, an active agent) into a soybean protein isolate (SPI). Different concentrations of CPE (0%, 10%, 12.5%, 15%, 17.5%, 20%, or 22.5%, w/w, based on SPI) were mixed into the films characterized by Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, thermogravimetry, tensile tests, a...