CASE REPORT A 74-year-old man developed a partial limbal stem cell deficiency secondary to a chemical injury in the left eye. He was treated with a sectorial conjunctival epitheliectomy and amniotic membrane transplantation. After 3 weeks there was an intact, smooth and stable corneal epithelium. DISCUSSION This case report emphasizes that conjunctival epitheliectomy and amniotic membrane tra...

We studied the paracellular permeability to mannitol of corneas with epithelium of corneal, limbal, or conjunctival origin. Corneas with epithelial defects reepithelialized by corneal or limbal epithelium were nonvascularized; the corneal permeability was initially increased and returned to normal 3 days later. When epithelial defects extended beyond the limbus, they were healed by conjunctival...

PURPOSE To establish whether fucoidin, by blocking the adhesion of leukocytes on the limbal vascular endothelium, prevents extravasation of the cells from the blood stream into the limbal stroma and the wounded area after corneal injury. Successful leukocyte blocking enabled investigation of the influence of leukocytes on corneal cellular proliferation after corneal wounding. METHODS Thirty-t...

introduction to evaluate the expression patterns of cytokeratin (k) 12, 13, and 19 in normal epithelium of the human ocular surface to determine whether k13 could be used as a marker for conjunctival epithelium. methods: total rna was isolated from the human conjunctiva and central cornea. those transcripts that had threefolds or higher expression levels in the conjunctiva than the cornea were ...

purpose: to evaluate the results of one 60-degree block conjunctival limbal autograft (clau) surgery in patients with unilateral total limbal stem cell deficiency (lscd). methods: five eyes of 5 patients were included. all patients had a history of chronic total unilateral lscd due to a chemical injury. two patients underwent one 60-degree block clau with amniotic membrane transplantation, two ...

The cornea requires constant epithelial renewal to maintain clarity for appropriate vision. A subset of stem cells residing at the limbus is primarily responsible for maintaining corneal epithelium homeostasis. Trauma and disease may lead to stem cell deficiency and therapeutic targeting to replenish the stemness capacity has been stalled by the lack of reliable corneal epithelial stem cell mar...

PURPOSE The corneal epithelium is essential for maintaining corneal transparency, and efforts have been made to develop improved techniques for corneal epithelial transplantation in patients with total limbal failure. We evaluated the suitability of transplanted cultivated human conjunctival epithelium (HCjE) as a corneal epithelium replacement in rabbits with total corneal and limbal deficienc...

PURPOSE To determine the expression and function of toll-like receptors (TLRs) in human conjunctival, limbal and corneal epithelial cells. METHODS Expression of TLRs was examined by real-time polymerase chain reaction, immunohistochemistry, and western blot analysis in human conjunctival, corneal and limbal epithelial cells and tissues. Ligand-stimulated nuclear factor kappaB activation; inte...

Long-term preservation of corneal limbal epithelium may decrease its quality and change the molecular signature of the limbal epithelial stem cells. In this study we have investigated the molecular profile of isolated corneal epithelial cells that have been in storage for an extended time. Isolated cells were characterised by the expression profile of different cytokeratins and markers of squam...

Corneal epithelium is maintained throughout life by well-orchestrated proliferation of limbal epithelial stem cells, followed by migration and maturation centripetally across the ocular surface. The present study sets out to explore the role tissue stiffness (compliance) may have in directing both differentiation and centripetal migration of limbal epithelial stem cells during homeostasis. For ...