Spermatozoa, especially those of the porcine species, are highly susceptible to in vitro chilling and ageing. Extenders are continuously developed to protect boar spermatozoa from chilling injury. New semen extenders and other modified preservation strategies require sensitive testing for essential sperm functions. The key process on the pathway of fertilization is capacitation. The aim of the ...

The aim of this study was to evaluate the effects of the addition of antioxidants (vitamin E, β- mercaptoethanol and their combination) to semen extender.  Progressive motility, viability and pH of fresh ram semen were examined. Ejaculate samples were collected with artificial vagina from three Ghezel-Merinose rams (3 years old) during non- breeding reproduction season. Semen was diluted with a...

Studies were conducted using 68 males from the seventh generation of a line of meat-type breeder chickens selected for increased duration of fertility of frozen-thawed semen and 69 males of the randomly selected control line. These chickens were used to determine the presence or absence of differences between the selected and control lines with respect to fertility and spermatozoal motility whe...

the aim of this study was to evaluate the effects of the addition of antioxidants (vitamin e, β- mercaptoethanol and their combination) to semen extender.  progressive motility, viability and ph of fresh ram semen were examined. ejaculate samples were collected with artificial vagina from three ghezel-merinose rams (3 years old) during non- breeding reproduction season. semen was diluted with a...

This study examined the effect of rapid cooling with air and CO2 on shell egg quality over 14 wk. The 240 fresh eggs were initially heated to 47 C for 24 h in an incubator, cooled using rapid air cooling or CO2 cooling, and then stored in air or CO2 in 250-mL jars for 14 wk. The CO2 levels were recorded of the jar atmosphere, of the egg air cell, and of the egg albumen. The Haugh units of each ...

An artificial insemination study was conducted on 17 female camels which were administered human Chorionic Gonadotrophin (hCG) to induce ovulation after confirming a follicle in the ovaries using sonography. The animals were inseminated with either diluted-cooled or fresh undiluted semen. No female camel could be impregnated with diluted and cooled semen, while pregnancy rate was low with neat ...

White rhinoceroses suffer from a low rate of reproduction in captivity. This study examines the role of male reproductive function as a contributing factor. We used ultrasonography to image accessory sex glands, testis and epididymis. Electroejaculation provided 36 ejaculates from 21 rhinoceroses. Based on the percentage of progressively motile spermatozoa, semen was categorized in three groups...

It was the aim of the present study to test whey as protective protein for the sperm cell in the long-term boar semen preservation medium TRIXcell. Analyses of sperm cell motility using computer-assisted semen analysis (CASA) indicated that the whey protein Porex has a similar protective effect as bovine serum albumin (BSA) in maintaining viability of stored boar sperm. Boar sperm diluted in TR...

The objective of this study was to evaluate the effect of reducing sperm numbers per insemination dose on fertility of cryopreserved buffalo bull semen. For this purpose, semen was collected at weekly intervals from a Nili-Ravi buffalo bull (Bubalus bubalis) using an artificial vagina in two batches. The ejaculates were split-sampled and diluted at 37°C with tris-citric acid extender having 15x...

the purpose of this study was to evaluate the probable effects of leptin addition in different levels to the semen extender on sperm quality (motility and motility parameters, viability, sperm membrane integrity, and dna damage). semen specimens were evaluated immediately after leptin addition, equilibration time and after thawing the frozen semen. five healthy buffalo bulls (5 ejaculates from ...