A recently introduced two-channel confocal microscope with correlated detection promises up to 50% improvement in transverse spatial resolution [Simon, Sergienko, Optics Express 18, 9765 (2010)] via the use of photon correlations. Here we achieve similar results in a different manner, introducing a triple-confocal correlated microscope which exploits the correlations present in optical parametr...

In light microscopy the transverse nature of the electromagnetic field precludes a strongly focused longitudinal field component, thus confining polarization spectroscopy and imaging to two dimensions (x,y). Here we describe a simple confocal microscopy arrangement that optimizes for signal from molecules with transition dipoles oriented parallel to the optic axis. In the proposed arrangement, ...

In this work we propose and build a multimodal optical workstation that extends a commercially available confocal microscope (Nikon Confocal C1-Si) to include nonlinear/multiphoton microscopy and optical manipulation/stimulation tools such as nanosurgery. The setup allows both subsystems (confocal and nonlinear) to work independently and simultaneously. The workstation enables, for instance, na...

The confocal laser microscope is a novel and interesting noninvasive tool for imaging skin lesions and subsurface skin lesions that are not visible to the naked eye or even by dermoscopy. Skin can be imaged in vivo or freshly biopsied (in vitro) skin specimens can be visualized immediately without the processing that is required for routine histopathology. Dynamic events (real time imaging) in ...

Non-contact optical techniques for 3D surface data acquisition like short coherence (white light) interferometry, optical profilers or fringe projection are well established for applications with low surface complexity, where the average surface roughness amplitude is small and neglectable compared to the form amplitudes or where the aspect ratios of amplitudes and spacings are small. In genera...

0278-6648/04/$20.00 © 2004 IEEE IEEE POTENTIALS Optical technologies, such as reflectance and fluorescence microscopy, may help detect and diagnose cancers that originate in the epithelium. The epithelium is the layer of tissue that is exposed to the environment and lines the body’s cavities. Cancers that originate in the epithelium include cervical, oral, colon, lung, stomach, bladder and skin...

We propose a new imaging method called hemispherical confocal imaging to visualize clearly a particular depth in a 3-D scene. The key optical component is a turtleback reflector which is a specially designed polyhedral mirror. To synthesize a hemispherical aperture, we combined the turtleback reflector with a coaxial camera and projector, to create on a hemisphere many virtual cameras and proje...

Confocal optical microscopy is a technique for increasing the contrast of microscope images, particularly in thick specimens. By restricting the observed volume, the technique keeps overlying or nearby scatterers from contributing to the detected signal. The price for this is that the instrument must observe only one point at a time (in the scanning laser version) or a group of separated points...

OBJECTIVE The accurate quantification of human diabetic neuropathy is important to define at-risk patients, anticipate deterioration, and assess new therapies. RESEARCH DESIGN AND METHODS A total of 101 diabetic patients and 17 age-matched control subjects underwent neurological evaluation, neurophysiology tests, quantitative sensory testing, and evaluation of corneal sensation and corneal ne...